Epstein-Barr virus (EBV) contributes to the MS pathogenesis because high levels of EBV EBNA 386–405 -specific antibodies cross react with the CNS-derived GlialCAM 370–389. However, it is unclear why only some individuals with such high autoreactive antibody titers develop MS.

2021年2月4日 · Here, we show that EBNA1 forms a cell cycle-dependent DNA crosslink with the EBV origin of plasmid replication oriP. EBNA1 tyrosine 518 (Y518) is essential for crosslinking to oriP and functionally required for episome maintenance and generation of EBV-transformed lymphoblastoid cell lines (LCLs).

Epstein–Barr nuclear antigen 1 (EBNA1) is a multifunctional, dimeric viral protein associated with Epstein–Barr virus (EBV). [1] . It is the only EBV protein found in all EBV-related malignancies. [2][3] It is important in establishing and maintaining …

Suspension-adapted CHO-K1 host cells were engineered to express the gene encoding Epstein-Barr virus (EBV) nuclear antigen-1 (EBNA-1) with and without the coexpression of the gene for glutamine synthetase (GS).

2008年3月1日 · The HEK293 cell line stably expressing the Epstein-Barr virus nuclear antigen-1 (HEK293-EBNA1, or 293E) is the most commonly used cell line for large-scale transfection. When using expression vectors bearing the Epstein-Barr virus origin of replication, oriP (such as the pTT vector), a threefold improvement in r-protein yield is generally ...

2018年2月20日 · The Epstein-Barr virus (EBV) nuclear antigen leader protein (EBNA-LP) is the first viral latency-associated protein produced after EBV infection of resting B cells. Its role in B cell transformation is poorly defined, but it has been reported to enhance gene activation by the EBV protein EBNA2 in vi …

2021年2月3日 · 近日，来自美国费城威斯达研究所的Paul M. Lieberman研究组在Cell杂志上发表了一篇题为Cell-cycle-dependent EBNA1-DNA crosslinking promotes replication termination at oriP and viral episome maintenance 的文章，揭示了EBNA1通过与DNA交联促进在oriP位点的复制终止实现附加体维持的具体分子机制。

Characteristics: This cell line is transfected with EBNA1t, a truncated version of EBV EBNA1 lacking the Gly-Gly-Ala repeats region. It has an enhanced ability to produce recombinant protein compared to HEK293-EBNA (Cellosaurus= CVCL_6974 ).

EBNA-3C acts as a transcriptional coregulator by interacting with various cellular and viral factors. For the last 10 years, we have been actively engaged in discerning the biological significance of these interactions and revealed that EBNA-3C primarily targets two important cellular pathways – cell cycle and apoptosis.

2022年1月24日 · Here we demonstrate high-affinity molecular mimicry between the EBV transcription factor EBV nuclear antigen 1 (EBNA1) and the central nervous system protein glial cell adhesion molecule...