Quantifying Golgi structure using EM: combining volume-SEM …
Light microscopy (LM) has been an effective tool for screening but fails to reveal fine details of Golgi structures such as vesicles, tubules and cisternae. Electron microscopy (EM) has …
Algorithm for Modern Electron Microscopic Examination of the Golgi …
Fast development of microscopic methods generates a huge difficulty for Golgi researchers to select the best protocol to use. Modern methods of light microscopy, such as super-resolution …
Golgi apparatus analyzed by cryo-electron microscopy
2013年8月18日 · In this review, we will focus on the influences of fixation on the architecture of Golgi apparatus, how it is seen by cryo-electron microscopy, as well as shedding light on what …
Novel scanning electron microscopy methods for analyzing …
2016年10月26日 · To overcome this problem, we have developed several novel scanning electron microscopy (SEM) methods for observing the 3D structure of subcellular organelles including …
Electron tomography of ER, Golgi and related membrane systems
2006年6月1日 · Electron tomography of cryofixed, freeze-substituted, and plastic-embedded samples allows three-dimensional visualization and display of dynamic, pleiomorphic …
Eukaryotes: Scanning Electron Microscopy of Intracellular Structures
1987年1月1日 · Scanning electron microscopy (SEM) is advantageous in the morphological research of intracellular structures that are spatially arranged intricatly. Advances in …
Subcellular Fraction Electron Microscopy - University of …
Subcellular fraction identification of RER, SER, Golgi, mitochondria, peroxisomes, melanosomes, etc. can be examined ultrastructurally using a pellet technique. Purified fractions are pelleted …
Unravelling the endoplasmic reticulum–Golgi intermediate
5 天之前 · The existence of an endoplasmic reticulum (ER)–Golgi intermediate compartment (ERGIC) in plant cells has long been debated. In our study we identified a dynamic Golgi …
Three-dimensional shape of the Golgi apparatus in different cell …
2015年11月24日 · In this study, we used serial section scanning electron microscopy (SEM) for the morphological study of the Golgi apparatus. This method is useful for three-dimensional …
Electron micrograph of the Golgi apparatus in an intestinal …
In these situations, ultrastructural studies (9,12) reveal triglyceride droplets within the SER but entry of nascent chylomicrons into the Golgi apparatus is apparently prevented, resulting in...