Bacteriophages for three strains of A. tumefaciens were concentrated by ultracentrifugation, stained with 1% phosphotungstic acid (PTA), or 0.5% uranyl acetate, and examined with the electron microscope. Phage PT11 was a bacillary-shaped particle with a whip-like tail containing a knob at its distal end.

Scanning electron microscope studies demonstrated that cells of Agrobacterium tumefaciens strains attach to cells on the cut surfaces of corn and wheat seedlings and to gladiolus disks. Bacterial cells attached to these monocots in the same manner as they attached to …

Here, we present a 3 Å cryoelectron microscopy (cryo-EM) structure of the T-pilus solved by helical reconstruction. Our structure reveals that the T-pilus is a stoichiometric assembly of the VirB2 major pilin and phosphatidylglycerol (PG) phospholipid with 5-start helical symmetry.

2023年5月8日 · To analyze the reaction of immature embryos infected, which seemed to provide the necessary by Agrobacterium tumefaciens in PureWheat, a combination of scanning electron microscopy (SEM), complete transcriptome analysis, and metabolome analysis was conducted to understand the progress.

1972年1月1日 · Agrobacterium tumefaciens strains 1D135 and B6 were characterized. Both strains formed circular, smooth, creamy, shiny colonies on rich medium. In the scanning electron microscope their morphologies were bacilliform rods which possessed 1 to 6 peritrichous flagella.

Using ultrathin-section transmission electron microscopy (TEM), freeze-fracture TEM (FFTEM), and energy-dispersive X-ray spectroscopy, we demonstrated that strain RH AL1 accumulates lanthanides...

Microscopy, Electron* Phosphotungstic Acid Rhizobium / cytology* Staining and Labeling Ultracentrifugation Uranium

There are several electron microscopy images of the bacterium on the Internet including this one, another, and another. Agrobacterium Protocols is a book on methods in molecular biology. Agrobacterium tumefaciens bacterial culture was provided by Dr. Steve Gleddie .

1970年3月1日 · Phage PIIBNV6 appeared to have an icosahedral head. The wide non-contractile tail terminated in a plate with pegs. Phage PIIBNV6-C was an icosahedral particle with a short, spike-like tail. Host cells of A. tumefaciens were encapsulated rods …

ELECTRON microscope studies of ultra-thin sections of the cells of several species of bacteria made by different workers have shown a regular structural pattern which can be summarized...