Early embryonic lethality (ie, before and up to mid-gestation) can be straightforward to analyze because the stage at which death occurs suggests why an embryo has failed. Here we summarize general strategies for analyzing early embryonic lethal phenotypes in genetically engineered mouse mutants.

Early embryonic lethality, i.e. before and up to mid-gestation, can be straightforward to analyze because the stage at which death occurs suggests why an embryo has failed. Here we summarize general strategies for analyzing early embryonic lethal phenotypes in genetically engineered mouse mutants.

2019年6月28日 · Using the one-step two-cell embryo microinjection (OSTCM) method, we generate viable adult chimeric mice with an embryonically lethal mutation of either Virma or Dpm1. These lethal mutations...

2024年8月13日 · In this study, we report the gross embryonic and perinatal phenotypes of 55 lethal strains randomly picked from a collection of mutants that carry piggyBac (PB) transposon inserts. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses suggested most of the analyzed mutations hit genes involved in heart and nervous ...

2011年12月6日 · Using the described basic pathology methods, along with provided histological atlas references and possible 3D imaging techniques, the pathologist can determine embryonic lethality with confidence and assist other biomedical …

1997年4月24日 · Inherited mutations in the human BRCA2 gene cause about half of the cases of early-onset breast cancer. The embryonic expression pattern of the mouse Brca2 gene is now defined and an interaction identified of the Brca2 protein with the DNA-repair protein Rad51.

To study essential maternal gene requirements in the early C. elegans embryo, we have screened for temperature-sensitive, embryonic lethal mutations in an effort to bypass essential zygotic requirements for such genes during larval and adult germline development. With conditional alleles, multiple essential requirements can be examined by ...

2024年12月5日 · In between these extreme cases, for species with more intermediate relatedness, D. albolineatus and D. margaritatus, F1 hybrids exhibit a mixture of lethality at embryonic, larval, and juvenile stages, as well as adult sterility. Overall, the range of observed phenotypes for given hybrids closely follows phylogenetic relatedness between the ...

2025年2月13日 · Early lethality (embryonic days 9.5-14.5) is almost always associated with severe placental malformations. Placental defects correlate strongly with abnormal brain, heart and vascular development ...

Embryonic and postnatal lethality limited the translational value of many KO mouse lines in the past three decades. The most promising method to rescue a lethal phenotype is the creation of mosaic mice by TALEN or CRISPR/Cas9 system.