2024年11月1日 · We generated sensory nerve EP4-specific knockout mice, with the genotype of Avil-CreEP4 fl/fl, was propagated. Transcriptome sequencing and bioinformatics analysis techniques were used to identify the potential molecular pathways involving …

Avil −/ mice by crossing Advillin-cre mice with EP4wt mice to validate of EP4 function in sensory nerves. qPCR analysis and immunostaining of EP4 confirmed that EP4 deletion was specifically in nerve (Supplementary Figure 5). Co-immunofluorescent staining of EP4 with CGRP showed that EP4 was expressed in bone sensory nerves, confirming ...

2021年8月2日 · We have identified PGE2/EP4-mediated skeletal interoception, in which PGE2 secreted by osteoblasts activates EP4 in sensory nerves to induce phosphorylation of cAMP-response element binding...

2024年3月5日 · To determine whether mechanical loading-induced bone formation is mediated by PGE2-EP4 signaling in sensory nerves, we generated sensory nerve-specific EP4 knockout mice (EP4 Avil –/–) by ...

2020年3月19日 · Here, we found that after sensory denervation, knockout of the EP4 receptor in sensory nerves, or knockout of COX-2 in osteoblasts, could significantly promote adipogenesis and inhibit osteogenesis in adult mice.

2022年1月27日 · EP4 expression in primary DRG neurons was increased more than 10-fold relative to treatment with Mg 2+ alone (Fig. 6a). We then generated EP4 Avil −/− mice by crossing Advillin-Cre mice with ...

2024年11月5日 · Previous research has shown that sensory nerve prostaglandin E2 receptor 4 (EP4) signaling not only governs pain perception but also influences bone formation. However, the relationship between sensory nerve EP4 and the pathogenesis of HO in the Achilles tendon remains unclear.

2020年5月12日 · In our study, we investigated whether EP4-PGE2 in sensory nerves regulate bone mass 86 accrual through direct control of commitment of MSCs by tuning down sympathetic nerve activity.

To determine whether mechanical loading-induced bone formation is mediated by PGE2-EP4 signaling in sensory nerves, we generated sensory nerve-specific EP4 knockout mice (EP4 Avil –/–) by crossing EP4 floxed (EP4 wt) mice with Advilin-Cre mice.

Here we report osteoblast secret prostaglandin E2 (PGE2) during aberrant subchondral bone remodeling induces pain and OA progression in mice. Specific deletion of the major PGE2 producing enzyme cyclooxygenase 2 (COX2) in osteoblasts or PGE2 receptor EP4 in peripheral nerve markedly ameliorates OA symptoms.