2023年5月1日 · Resistin-like molecule (Relmβ), a goblet cell-derived mediator, can in turn act on goblet cells and enhance MUC2 secretion via MAPK/ERK axis [127]. Capsaicin (CAP) promotes MUC2 secretion by activating transient receptor potential vanilloid 1 (TRPV1), a non-selective cation channel which is broadly expressed on sensory neurons in respiratory ...

2002年9月6日 · In this study we show that PMA stimulates expression of the MUC2 gene in HM3 cells through activation of PKC. Such MUC2 up-regulation is Ras- and Raf-dependent, requires activation of the MEK/ERK signaling pathway, and ultimately involves activation of a nuclear factor, NF-κB. TriReagent and PMA were obtained from Sigma.

2020年6月11日 · 摘要: 哺乳动物肠道分泌大量黏液形成黏液层，其主要成分是黏蛋白2（MUC2）。 黏液层对肠道的润滑、保护及强大的屏障功能依赖于MUC2自身的特殊网状结构、性质与功能。 肠道MUC2的存在对维系肠腔微环境稳定和肠上皮细胞功能正常具有重要作用。 本文综述了肠道MUC2的分泌、结构、合成调控及其在肠道疾病发生发展中的作用，为经调控MUC2防治肠道疾病提供理论依据。 Corresponding author: WEN Zhengshun, associate professor, E …

在这项研究中，我们假设由 MUC2 生物合成和产生诱导的 ER 应激会增强杯状细胞的促炎反应。 该研究的目的是：1) 确定 WT 和 CRISPR/Cas9 MUC2 KO LS174T 人杯状细胞中的促炎反应和 2) 确定 MAPK 信号通路在 WT 和 MUC2 KO 杯状细胞中改变促炎反应的途径。

2010年1月11日 · These data indicate that the signaling pathways associated with the inflammatory response can modulate the expression of MUC2 and MUC4 intestinal mucin genes, in human and mouse gastric tumors....

2002年9月6日 · Phorbol 12-myristate 13-acetate (PMA) treatment of colon cancer cell lines increases MUC2 expression, so we have undertaken a detailed analysis of the effects of PMA on the promoter activity of the 5'-flanking region of the MUC2 gene using stably and transiently transfected promoter reporter vectors.

2025年3月22日 · MUC2 mucin biosynthesis induces ER stress in colonic goblet-like cells driven by reactive oxygen species. To determine if endoplasmic reticulum (ER) stress induced by high MUC2 mucin biosynthesis can affect the cells’ ability to produce pro-inflammatory cytokines, wildtype (WT) and a CRISPR-Cas9 gene-edited MUC2KO LS174T goblet-like cell ...

Quercetin stimulation of LS174T cells increases phosphorylation levels of extracellular signal regulated kinase (ERK)1-2 and protein kinase C (PKC) α and the induction of MUC2 and MUC5AC secretion and mRNA relies on phospholipase C (PLC), PKC, and ERK1-2 signaling pathways since the PLC inhibitor U73122, the PKC inhibitor bisindolylmaleimide ...

2020年4月9日 · In this study, we investigated the stimulatory effects of exogenous nicotinamide adenine dinucleotide (NAD+) on the expression of MUC2 in LS 174T goblet cells. Methods: Genes related to MUC2 synthesis were measured by quantitative real-time PCR (qPCR).

Ro31-8220 (PKC inhibitor) inhibited IL-1beta-mediated MUC2 gene expression and mucin secretion. It inhibited ERK phosphorylation, but did not inhibit p38 phosphorylation. LY294002 (PI3K inhibitor) also suppressed MUC2 expression, but did not inhibit any MAPKs phosphorylation.