Tangential flow filtration (TFF) is a rapid and efficient method for separation and purification of biomolecules. It can be applied to a wide range of biological fields such as immunology, protein chemistry, molecular biology, biochemistry, and microbiology. TFF can be used to concentrate and desalt sample solutions ranging in volume from 10 mL to

單次使用 TFF (ex：Pellicon ®? Capsules）消除了傳統 TFF 過濾製程的許多步驟，從而在過濾 mAbs 和其他生物製劑時降低製程複雜性、節省時間並節約資源。 節省時間：一次性使用 TFF 省去了產品處理前後的清洗步驟，提高了設備產能。

Downstream bioprocessing involves many steps (ex: clarification, chromatography, filtration, etc.) to purify and concentrate a desired molecule produced by natural sources. One of these methods, tangential flow filtration (TFF), is used in downstream bioprocessing to both:

Pellicon® Capsules are the ideal single-use TFF device and provide ease-of-use, process flexibility, rapid batch turnaround, reduced risks and easier validation. Our innovative capsules have a holderless design and are provided gamma sterilized and wetted with purified water for easy installation and reduced pre-use steps,

We describe a scalable workflow for the isolation of exosome extracellular vesicles using tangential flow filtration (TFF) and size exclusion chromatography (SEC).

2023年7月2日 · 偏向流过（Tangential Flow Filtration，TFF）是一种过滤过程，过滤液在接触滤膜的同时流动。 它与“横向流过”或“常规过滤”相对立，液体在这里沿着膜上流动并通过膜下方。

切向流过滤 （TFF）是一种有用的方法，可用于按大小分离液体溶液中的分子或颗粒，并且能够实现浓缩和改变缓冲液组分的能力。 TFF几乎用于所有生物制药生产下游工艺中，以提供温和、快速的浓缩和透析（去除或分离成分的稀释工艺）。 相比之下， 凝胶过滤层析 按大小分离分子，并提供改变缓冲液成分的能力，但这种技术不能浓缩目标实体。 在 普通过滤 （NFF）中，固体通常在膜上形成滤饼或第二层。 在处理生物分子或更小的颗粒时，即使是膜上的第二层薄层通常也 …

2018年12月16日 · This study reports the use of tangential flow filtration (TFF) for the highly efficient isolation of EVs from large volumes of samples. When compared to ultracentrifugation (UC), which is the most widely used method to concentrate EVs, TFF is a more efficient, scalable, and gentler method.

• Understand the main applications of TFF in large scale API manufacturing. • Perform a bench scale TFF operation on separation and concentration to understand the important operation principles. • Understand the critical parameters associated with large scale TFF operation. • Perform basic trouble shooting methods in TFF operation.

UniFlux is a fully automated tangential flow filtration/cross flow filtration systems for clarification, concentration and diafiltration applications in pilot to manufacturing scale. Available in 4 sizes 10, 60 and 120 Lpm, for pilot to production-scales (400 Lpm version as a custom product). Maximizes productivity in tangential flow filtration.