2022年4月13日 · ExbB and ExbD are cytoplasmic membrane proteins that associate with TonB to convey the energy of the proton-motive force to outer membrane receptors in Gram-negative bacteria for iron uptake. The...

2019年2月5日 · TonB复合物由TonB、ExbB、ExbD构成，是革兰氏阴性菌对外界营养物质主动转运过程的能量提供单位，在革兰氏阴性菌分布广泛。 近年来，对TonB-ExbB-ExbD复合物的功能、结构及作用机制取得了重大研究进展，然而此复合物在不同的细菌也存在功能及作用机制上的差异。

2019年10月4日 · The TonB–ExbB–ExbD molecular motor harnesses the proton motive force across the bacterial inner membrane to couple energy to transporters at the outer membrane, facilitating uptake of ...

大肠杆菌中的exb基因座由两个基因组成，分别称为exbB和exbD。Exb功能与TonB功能相关，因为大多数与TonB相关的过程都由Exb增强。像tonB突变体一样，exb突变体对大肠菌素M和阿波霉素具有抗性，但与tonB突变体相比，只显示出对大肠菌素B和D的敏感性降低。

Energy is derived from the proton motif force (pmf) of the cytoplasmic membrane (CM) through the action of three proteins, namely, TonB, ExbB, and ExbD, which are located in the CM and extend into the periplasm. The leaky phenotype of exbB exbD mutants is caused by partial complementation by homologous tolQ tolR.

1993年3月15日 · Using ExbB-B-lactamasefusion proteins as reporters for a periplasmic versus a cytoplasmic location of the fusion sites, and accessibility of ExbB in spheroplasts and cell lysates to aminopeptidase K, trypsin, and proteinase K, we arrived at a model of ExbB topology in the cytoplasmic membrane.

2010年10月12日 · Earlier studies showed that ExbB is the most abundant protein of the ExbB-ExbD-TonB complex and stabilizes TonB and ExbD. To advance understanding of the role of ExbB in the membrane organization of the ExbB-ExbD-TonB complex, His-tagged ExbB was solubilized with decyl maltoside and purified to electrophoretic homogeneity.

TonB protein appears to couple the electrochemical potential of the cytoplasmic membrane to active transport across the essentially unenergized outer membrane of gram-negative bacteria. ExbB protein has been identified as an auxiliary protein in this process. In this paper we show that ExbD protein, …

2013年12月1日 · Uptake of ferric siderophores, vitamin B12, and other molecules in gram-negative bacteria is mediated by a multi-protein complex known as the TonB system. The ExbB and ExbD protein components of the TonB system play key energizing roles and are homologous with the flagellar motor proteins MotA and MotB.

2014年5月6日 · We copurified ExbB with ExbD as an ∼240 kDa protein-detergent complex, measured by light scattering and by native gels. Quantitative Coomassie staining revealed a stoichiometry of ExbB 4 -ExbD 2. Negative stain electron microscopy and 2D analysis showed particles of ∼10 nm diameter in multiple structural states.