2018年5月15日 · To determine how Dnmt3a and Dnmt3b influence B cell activation and differentiation, the gene expression program was determined for B220 + GL7 − Fas − naive follicular B cells (nB) from the ...

2011年11月21日 · On FACS, gate on B cells positive for GL7 and CD95 (or Fas), it works quite nice. Got a technical question? Get high-quality answers from experts.

Here we identified Sia-dependent epitope specificity for GL7, a rat monoclonal antibody, to probe germinal centers upon T cell-dependent immunity. GL7 recognizes sialylated glycan(s), the α2,6-linked N-acetylneuraminic acid (Neu5Ac) on a lactosamine glycan chain(s), in both Sia modification- and Sia linkage-dependent manners. In mouse germinal ...

Download scientific diagram | (A) Germinal center B cells (GL7+Fas+ gated, total B cell gate shown) in C57BL/6 (B6) or IL-21−/− mice treated with anti-IL-6 (αIL6) or isotype mAb.

2011年9月6日 · Expression of Fas, GL7, CD38, and PNA ligand was analysed for CD138 − gated cells. Numbers indicate the percentages of cells in the indicated quadrants or windows.

2020年12月1日 · We first added ascorbic acid at the beginning of the first stage and replenished the culture with fresh ascorbic acid every day till the end of the second stage. Under such conditions, ascorbic acid had little effect on the differentiation of iGCB cells, as determined by GL7 and FAS staining at the end of the first stage (Figures 1 B and 1C

2017年10月12日 · Murine B cell populations from immunized mice and organoid and 2D cultures were purified by FACS using FAS − GL7 − IgD + B220 + splenocytes as naive B cells, and FAS + GL7 + B220 +...

2008年10月17日 · Fas is highly expressed in activated and germinal center (GC) B cells but can potentially be inactivated by misguided somatic hypermutation. We employed conditional Fas-deficient mice to investigate the physiological functions of Fas in various B cell subsets.

为了表征处于中间、GC前状态的活化B细胞，该研究团队检测了IgD、CD38、CD95、GL7、活化诱导的胞苷脱氨酶（AID）和细胞分裂之间的关系。用CellTrace Violet（CTV）标记4-羟基-3-硝基苯基乙酰基（NP）特异性B1-8hi AicdaGFP/+B细胞，并将其过继转移到小鼠体内。

Invitrogen Anti-GL7 Monoclonal (GL-7 (GL7)), eBioscience™, Catalog # 14-5902-82. Tested in Immunohistochemistry (Frozen) (IHC (F)), Flow Cytometry (Flow) and Immunoprecipitation (IP) applications. This antibody reacts with Human, Mouse samples. Supplied as 100 µg purified antibody (0.5 mg/mL).