Feline immunodeficiency virus (FIV) is one of the most common and consequential infectious diseases of cats around the world. In infected cats, FIV attacks the immune system, leaving the cat vulnerable to many other infections.

The Molecular Diagnostics Laboratory at Auburn University has developed a rapid, highly sensitive and specific quantitative PCR approach targeting the most conserved capsid (gag) gene. The PCR detects as few as single copies of the integrated viral DNA as well as the RNA genome or gag mRNA of FIV subtypes A, B and C. (Wang et al., 2010b). By ...

1999年3月1日 · An improved quantitative polymerase chain reaction (qPCR) method based on a combination of real-time detection and the 5′–3′ nuclease activity of the Taq DNA polymerase was developed to quantify the provirus load of feline immunodeficiency virus (FIV), a lentivirus of veterinary importance and an animal model for AIDS research. Two ...

PCR (Polymerase Chain Reaction) The PCR test actually tests for the DNA of the virus itself, so can be used for kittens as well as adults; it can also be used at an earlier stage after infection, as it does not need to wait for the antibodies to form (which can take several weeks).

Diagnosis of feline immunodeficiency virus (FIV) infection by polymerase chain reaction (PCR) has recently become available, but little is known about the performance of this assay. The purpose of this study was to determine the sensitivity and specificity of …

Feline immunodeficiency virus (FIV) proviral DNA was detected by the polymerase chain reaction method (PCR). PCR products were detected by gel electrophoresis and ethidium bromide staining. The P-10, P-15 and P-24 regions of the gag gene of FIV were chosen as the target sequences for amplification, …

By detecting RNA produced by actively replicating as well as integrated DNA, this PCR offers 10-1,000-fold increased detection over detection of genome-integrated FIV DNA only combined with essentially 100% specificity. Based on known target sequences, subtypes D and E will also be detected, but these targets have not been tested.

2016年6月1日 · We recently showed that two immunochromatography point-of-care FIV antibody test kits (Witness FeLV/FIV and Anigen Rapid FIV/FeLV) were able to correctly assign FIV infection status, irrespective of FIV vaccination history, using …

FeLV and FIV are both RNA viruses carrying the enzyme reverse transcriptase, which, after infecting a cell, makes a DNA copy of the viral RNA. The DNA copy is inserted into the host’s DNA and is called a. “provirus” or “proviral DNA.” Cats can be positive on FeLV proviral DNA PCR but not actually be infectious to other cats.

2012年4月1日 · The objectives of the current study were to estimate the sensitivity and specificity of three real-time polymerase chain reaction (PCR) tests for diagnosis of feline immunodeficiency virus (FIV) infection in domestic cats, both individually and when interpreted in series with one of two serological …