利用Cre/LoxP和来自酵母的Flp/FRT系统可以研究特定组织器官或特定细胞中靶基因灭活所导致的表型。 通过常规基因打靶在基因组的靶位点上装上两个同向排列的loxp，并以此两侧装接上loxp的（loxP floxed）ES细胞产生“loxP floxed”小鼠。

In the Flp-In System, three different vectors are used to generate isogenic stable mammalian cells lines expressing your gene (s) of interest. The first major component of the Flp-In™ System is the pFRT/lacZeo target site vector that is used to generate a Flp-In host cell line.

Jun 6, 2019 · We developed a loop-mediated isothermal amplification (LAMP)–fluorescent loop primer (FLP) assay for genotyping the A/G 2254 single nucleotide polymorphism (SNP) in the viral DNA polymerase gene of species Equid alphaherpesvirus 1 (EHV-1), which is associated with the neuropathogenic potential of this virus. In addition to the use of regular ...

The Flp-In™ System streamlines the generation of stable mammalian expression cell lines by taking advantage of a Saccharomyces cerevisiae -derived DNA recombination system.

Flp-In™表达会在您选择的哺乳动物细胞系基因组中引入一个Flp重组靶标（FRT）位点。 随后，包含您感兴趣的基因的表达载体会通过FRT位点的Flp重组酶介导DNA重组，整合入基因组。

Mar 28, 2018 · Our collection allows tetracycline-inducible expression of proteins with various tags suitable for protein localization, FRET, bimolecular fluorescence complementation (BiFC), protein dynamics studies (FRAP), co-immunoprecipitation, the RNA tethering assay and cell sorting.

Dec 19, 2017 · Here we established a recombinase-mediated cassette exchange (RMCE) protocol for the fast and effective, drug-free isolation of recombinant cells. Distinct fluorescent protein patterns identified...

We developed a loop-mediated isothermal amplification (LAMP)–fluorescent loop primer (FLP) assay for genotyping the A/G single nucleotide polymorphism (SNP) in the viral DNA polymerase gene of species Equid 2254 alphaherpesvirus 1 (EHV-1), which is associated with the neuropathogenic potential of this virus.

Jul 1, 1998 · Improved FLP recombinases were identified through cycles of increasing stringency imposed by both raising temperature and reducing protein expression, combined with repetitive cycles of...

Our Flp-In™ Complete System allows integration and expression of your gene of interest in mammalian cells at a specific genomic location. The Flp-In System involves introduction of a Flp Recombination Target (FRT) site into the genome of the mammalian cell line of choice.