Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only particular parts of a nucleic acid sequence with a high degree of sequence complementarity.

2022年3月24日 · 荧光原位杂交（Fluorescence in situ hybridization FISH）是一门新兴的分子细胞遗传学技术，是 20 世纪 80 年代末期在原有的放射性原位杂交技术的基础上发展起来的一种非放射性原位杂交技术。

Fluorescence in situ hybridization (FISH) is the most convincing technique for locating the specific DNA sequences, diagnosis of genetic diseases, gene mapping, and identification of novel oncogenes or genetic aberrations contributing to various types of cancers.

荧光原位杂交（Fluorescence in situ hybridization，FISH）是20世纪80年代末在放射性 原位杂交技术 基础上发展起来的一种非放射性 分子生物学 和 细胞遗传学 结合的新技术，是以荧光标记取代 同位素标记 而形成的一种新的原位杂交方法。 [1] 荧光原位杂交技术 问世于20世纪70年代后期。 1977年，荧光标记的抗体被应用于识别特异性DNA—RNA杂交I I。 1980年，J.G.Baunlan等将应用化学偶联的方法将荧光素结合到 RNA探针 上用于直接快速的特异性靶序列检测。 [2]

2024年4月22日 · Fluorescence in situ hybridization (FISH) is a technique pathologists use in genetic testing to help diagnose diseases caused by chromosomal differences. Healthcare providers can also use FISH to detect gene changes that they can target to treat cancer.

荧光原位杂交 （FISH） 是一种使用仅与染色体的那些部分高度序列互补性结合的 荧光探针 的分子细胞遗传学技术。 它是由生物医学研究人员在20世纪80年代初开发的，用于检测和定位染色体上特定DNA序列的存在与否。 荧光显微镜 可用于找出荧光探针与染色体结合的位置。 FISH通常用于发现DNA中的特定特征，用于遗传咨询，药物和物种鉴定。 FISH也可用于检测和定位细胞，循环肿瘤细胞和组织样品中特定的RNA靶标（mRNA，lncRNA和miRNA）。 一、试剂配制. 1.变 …

3 天之前 · Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that allows the localization of a specific DNA sequence or an entire chromosome in a cell. It is utilized to diagnose genetic diseases, gene mapping, and identification of chromosomal abnormalities, and may also be used to study comparisons among the chromosomes ...

2020年8月16日 · Fluorescence in situ hybridization (FISH) provides researchers with a way to visualize and map the genetic material in an individual's cells, including specific genes or portions of genes. This may be used for understanding a variety of chromosomal abnormalities and other genetic mutations.

2020年9月9日 · RNA fluorescence in situ hybridization (FISH) and antibody staining/immunofluorescence (IF) are widely used to detect distributions of mRNAs and proteins. Here...

Fluorescence in situ hybridization (FISH) is a standard technique used in routine diagnostics of genetic aberrations. Thanks to simple FISH procedure is possible to recognize tumor-specific abnormality. Its applications are limited to designed probe type.