Nov 1, 1996 · Roles for FabA and FabZ in fatty acid biosynthesis. Both FabA and FabZ dehydratases function interchangeably in the cycles of fatty acid elongation up to 10 carbons. FabA is more active in the dehydration of β-hydroxydecanoyl-ACP, although FabZ also functions in …

Nov 22, 2016 · We present here a 2.55 Å crystal structure of type II FAS dehydratase FabZ in complex with holo-ACP, which exhibits a highly symmetrical FabZ hexamer-ACP3 stoichiometry with each ACP binding to...

在这项研究中，我们对这种酶进行了表征，我们将其称为厌氧氨氧化特异性 FabZ（“amxFabZ”），以研究梯烷脂质未解决的生物合成途径。 我们发现 amxFabZ 显示出与“经典”FabZ 明显的序列差异，例如底物结合隧道内部的大体积非极性残基，其中经典酶具有甘氨酸。

FabA和FabZ表现出广泛的重叠链长特异性。 FabZ脱水酶可有效催化短链β-羟酰基ACP和长链饱和和不饱和β-羟酰基ACP的脱水。 FabA对中链长度的β-羟酰基-ACP最为活跃，并且对短链和长链饱和的β-羟酰基-ACP都具有显着的活性。

May 1, 2023 · In this study, we characterize this enzyme, which we call anammox-specific FabZ (“amxFabZ”), to investigate the unresolved biosynthetic pathway of ladderane lipids.

Feb 29, 2008 · β-Hydroxyacyl-acyl carrier protein dehydratase (FabZ) is an important enzyme for the elongation cycles of both saturated and unsaturated fatty acids biosyntheses in the type II fatty acid biosynthesis system (FAS II) pathway. FabZ has been an essential target for the discovery of compounds effective against pathogenic microbes.

Feb 23, 2016 · To this end, we have cloned, expressed, and purified the β-hydroxyacyl-acyl carrier protein dehydratase (FabZ) from both Francisella tularensis (FtFabZ) and Yersinia pestis (YpFabZ). We also solved the crystal structures and performed an enzymatic characterization of both enzymes and several mutant forms of YpFabZ.

Oct 2, 2020 · FabZ is a 17kD beta-hydroxyacyl-acyl carrier protein (ACP) dehydratase that primarily catalyzes the dehydration of beta-hydroxyacyl-ACP to trans-2-acyl-ACP, the third step in the elongation phase of the bacterial/ plastid, type II, fatty-acid biosynthesis pathway.

There are two genes, fabA and fabZ, encoding β-hydroxyacyl-acyl carrier protein (ACP) dehydratases that function in the dissociated, type II fatty acid synthase system of Escherichia coli. We have investigated their roles in fatty acid synthesis by purifying the two proteins and reconstituting cycles of fatty acid synthesis in vitro using five ...

Oct 2, 2020 · FabZ is the primary dehydratase that participates in the elongation cycles of saturated as well as unsaturated fatty acid biosynthesis, whereas FabA is more active in the dehydration of beta-hydroxydecanoyl-ACP. The FabA structure is homodimeric with two independent active sites located at the dimer interface.