2021年1月24日 · Fatty acid transport 1 (FATP1) is an evolutionarily conserved protein that localizes to the plasma membrane to enhance the transportation of fatty acids (FAs). In line with this function, FATP1 is involved in the metabolism of …

Fatty acid transport proteins (FATPs) are an evolutionary conserved family of integral membrane proteins found at the plasma membrane and on internal membranes. FATPs facilitate the unidirectional uptake and/or intracellular activation of unesterified long-chain and very long-chain fatty acids into a variety of lipid-metabolizing cells and tissues.

2006年3月1日 · We demonstrate for the first time that FATP1 and ACSL1 coimmunoprecipitate in 3T3-L1 adipocytes, indicating that these proteins form an oligomeric complex. The efficiency of FATP1 and ACSL1 coimmunoprecipitation is unaltered by acute insulin treatment, which stimulates fatty acid uptake, or by treatment with isoproterenol, which decreases fatty ...

2006年12月14日 · 使用稳定的fatp1或fatp4敲低（kd）3t3-l1细胞系（源自逆转录病毒递送的短发夹rna（shrna））研究了脂肪酸转运蛋白1（fatp1）和fatp4在促进脂肪细胞脂肪酸代谢中的作用。

2009年9月18日 · FATP-dependent production of AMP was evaluated using FATP4 proteoliposomes, and fatty acid-dependent activation of AMP-activated protein kinase (AMPK) was assessed in 3T3-L1 adipocytes. Insulin-stimulated fatty acid influx (palmitate or arachidonate) into cultured adipocytes resulted in an increase in the phosphorylation of AMPK and its ...

2003年7月11日 · Fatty acid transport proteins (FATPs/solute carrier family 27) are integral transmembrane proteins that enhance the uptake of long-chain and very long chain fatty acids into cells. In humans FATPs comprise a family of six highly homologous proteins, hsFATP1–6, which are found in all fatty acid-utilizing tissues of the body.

FATP1 was the first FATP to be identified and is highly expressed in adipose tissue, skeletal muscle and, to a lesser extent, in heart. FATP2 is predominantly expressed in liver and kidney cortex. FATP3 shows a broader expression pattern with high mRNA and protein levels in lung.

2012年9月14日 · Here, we used a gain-of-function approach and established FATP1, ACSVL4/FATP4 and ACSL1 stably expressing 3T3-L1 adipocytes by retroviral transduction. All overexpressing cell lines showed increased acyl-CoA synthetase activity and fatty acid uptake.

2011年9月2日 · We show that TR4 facilitates lipid accumulation in 3T3-L1 adipocytes via induction of the FATP1 gene. Further study showed that TR4 transactivated FATP1 5′ promoter activity via direct binding to the TR4 responsive element located at the FATP1 5′ promoter region.

A cDNA encoding a novel fatty acid transport protein (FATP) was identified recently using expression cloning methodologies. We have studied the expression of FATP in differentiating 3T3-L1 cells and adipose tissue in vivo. When 3T3-L1 preadipocytes are treated with a combination of methylisobutylxan …