
Fatty acid transport protein 2 reprograms neutrophils in cancer
2019年4月17日 · PMN-MDSCs had a much higher expression of Slc27a2, which encodes FATP2. This was confirmed by quantitative PCR (qPCR) (Fig. 1a). No upregulation of other transporters and receptors involved in...
Profiling the expression of fatty acid-binding proteins and fatty …
2021年8月1日 · RT-qPCR and WB were performed to assess the knockdown efficiency of FABP3, FABP4, and FABP5 siRNA at the mRNA and protein level, respectively. After a 48-hour siRNA treatment (which did not impact on cell viability), FABP mRNA was reduced by 80.3% (FABP3), 92.3% (FABP4), and 77.5% (FABP5), relative to the AllStar negative control ( Fig. 4 A- 4 C).
Fatty acid transport proteins (FATPs) in cancer - PubMed
FATPs (fatty acid transport proteins) are a group of proteins involved in fatty acid uptake, mainly localized within cells and the cellular membrane, and have a key role in long-chain fatty acid transport. FATPs are composed of six isoforms that are tissue-specific and encoded by a …
Unraveling FATP1, regulated by ER-β, as a targeted breast cancer ...
2019年10月1日 · Recently, our team has shown that FATP1 has a pivotal role in FA transfer between breast cancer cells (BCCs) and non-cancerous cells in the microenvironment. We aimed to investigate the role of...
Interaction between CD36 and FABP4 modulates adipocyte-induced fatty ...
2021年9月24日 · We showed that CD36 directly interacts with FABP4 to regulate fatty acid import, transport, and metabolism. CD36 and FABP4 inhibition induces apoptosis in tumour cells. These results indicate that...
FATP2 (SLC27A2) Human qPCR Primer Pair (NM_003645)
A ready-to-use mix for SYBR Green qPCR, containing ROX reference and suitable for all qPCR instruments without adjusting the concentration of ROX. 4 x 1.25 ml (500 rxns/20ul reaction)
FATP | MCE 生命科学试剂服务商 - MCE-生物活性分子大师
FATP 1-IN-2 (compound 12a) 是芳基哌嗪的衍生物,是一种具有口服活性的脂肪酸转运蛋白1 (FATP1) 抑制剂 (对人和小鼠的 IC50 值分别为 0.43 μM 和 0.39 μM)。 Lipofermata 是一种脂肪酸转运蛋白 2 (FATP2) 抑制剂。 Lipofermata 在 Caco-2 细胞中显示脂肪酸转运抑制,IC 50 为 4.84 μM。 Lipofermata 是一种 spiro-indoline-thadiazole 类似物,具有锌抑制的抗菌活性。 Lipofermata 对 E.coli K-12 中锌的稳态有干扰作用,MIC 为 16 μM。
探针法快速qPCR预混液Fast Probe qPCR mix (or with UNG) - Takara
2025年3月1日 · 本制品是采用探针法进行Real Time PCR(qPCR)检测的快速试剂。 通过对添加了抗体的Hot Start PCR酶和Real Time PCR用Buffer分别进行了改良,可降低引物二聚体出现频率,具有扩增特异性高、检测灵敏度高等特点,实现快速PCR反应和在宽广的动态定量区域内对靶基因进行准确定量、检测,重复性好,可进行可信度高的Real Time PCR分析。 本制品在2×Premix试剂中已添加了Tli RNaseH(耐热性RNaseH),以cDNA为模板进行PCR反应时, …
实时荧光定量PCR(RT-qPCR) - 知乎专栏
实时荧光定量 PCR (RT-qPCR) 是一种用于实时扩增和检测特定 DNA 或 RNA 序列的分子生物学技术。 该方法利用荧光染料或探针,当与扩增的 DNA 或 RNA 分子结合时会发出信号,从而可以对目标序列进行量化。 RT-qPCR 通常用于基因表达分析、病毒载量定量和基因突变检测等应用。 使用 逆转录酶 和特定引物将RNA样品反转录成cDNA。 该步骤将RNA模板转化为更稳定且可扩增的DNA形式。 反转录过程中使用特定引物。 2 PCR扩增: 使用特定引物对cDNA进行PCR扩 …
qPCR技术介绍(一)——基础概念 - Takara
2023年3月30日 · Real Time PCR,即实时荧光定量PCR,也被称为定量PCR或qPCR,是实时监测PCR扩增产物并进行解析的方法。 由于定量PCR具有操作简单、快速方便、灵敏度高、重复性好、污染率低等优点,被广泛应用于医学检测、药物疗效考核、基因表达研究、转基因研究、基因 …
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