2021年8月1日 · RT-qPCR and WB were performed to assess the knockdown efficiency of FABP3, FABP4, and FABP5 siRNA at the mRNA and protein level, respectively. After a 48-hour siRNA treatment (which did not impact on cell viability), FABP mRNA was reduced by 80.3% (FABP3), 92.3% (FABP4), and 77.5% (FABP5), relative to the AllStar negative control ( Fig. 4 A- 4 C).

2019年10月1日 · Recently, our team has shown that FATP1 has a pivotal role in FA transfer between breast cancer cells (BCCs) and non-cancerous cells in the microenvironment. We aimed to investigate the role of...

2016年7月1日 · FATP1-mediated activation of fatty acids is a novel approach to limit inflammation. Fatp1 deficiency primed macrophages for pro-inflammatory activation. Lack of Fatp1 led to greater HFD-induced adipose inflammation. Fatp1−/− adipose tissue macrophages were classically activated.

FATP1 enhances the proliferation and promotes the adipogenic trans-differentiation of C2C12 cells. (A) The mRNA expression levels of PPARG and FATP1 as determined by RT-qPCR. (B) Histogram showing the quantitation of oil red O staining. (C) The triglyceride levels of C2C12 cells on day 8 of adipogenic trans-differentiation.

In the present study, FATP1 mRNA was mainly detected in heart and skeletal muscle, whereas its expression level was relatively low in adipose tissue ( Figure 1A). Indeed, it has been reported...

2018年2月1日 · Fatty acid uptake through FATP1 regulates the inflammatory cytokines through ceramide and JNK signaling in the macrophages. An inhibitor of FATP1 attenuated the production of inflammatory cytokines in an LPS-induced lung injury in vivo mouse model. Macrophages are important cells that need to be controlled at the site of inflammation.

Using 293 T-REx cell lines that stably express FATP1 or FATP4 from a tetracycline-inducible promoter, this work evaluated gene expression of key genes involved in fatty acid metabolism using QPCR, protein expression of FATP1 and FATP4 using Western blots, and fatty acid transport to address the roles of these two FATP isoforms in fatty acid ...

(A) qPCR analysis of FATP1 mRNA expression (human and mouse) in RPE of young (1-3-month-old, n = 4) and aged (6-9-month-old, n = 6) wild type (WT) and hFATP1 transgenic (TG) mice. Results are...

2024年11月12日 · Expression of Fatp1 (A) and Fatp4 (B) in the NR and RPE of Fatp1 +/+ and Fatp1 −/− mice as determined by qPCR. Results are expressed as a percentage of Actin mRNA expression for NR and as a percentage of Mertk mRNA expression for RPE. mRNA was extracted from tissue pools of 12 Fatp1 +/+ and 10 Fatp1 − / − mice aged 5 months old.

2022年7月11日 · FATP1 enhances the proliferation and promotes the adipogenic trans-differentiation of C2C12 cells. (A) The mRNA expression levels of PPARG and FATP1 as determined by RT-qPCR. (B) Histogram showing the quantitation of oil red O staining. (C) The triglyceride levels of C2C12 cells on day 8 of adipogenic trans-differentiation.