Formate dehydrogenase H (Fdh-H) is encoded by the fdhF gene located at 92 min on the E. coli chromosome. The 80-kDa selenopolypeptide is part of the formate-hydrogen lyase complex and either is located in the cell cytoplasm or is loosely associated with the inner surface of the cytoplasmic membrane.

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Selenylated truncated polypeptide chains or beta-galactosidase fusion proteins were synthesized when the deletion clones or gene fusions, respectively, contained the fdhF gene fragment coding for the selenopolypeptide sequence from amino acid residue 129 to amino acid residue 268.

Figure 3 shows the structures of the Mo‐ or W‐containing subunits for the four enzymes that have to date been crystallographically characterized: the E. coli FdhF formate dehydrogenase (Mo, SeCys), 7 the FdnG subunit of the E. coli FdnGHI formate dehydrogenase (Mo, SeCys), 22 the FdhA subunit of the D. gigas FdhAB formate dehydrogenase (W, SeCys...

2008年2月26日 · Repressed by oxygen, nitrate, nitrite, and other electron acceptors. Consists of two separable enzymatic activities: a formate dehydrogenase component (FDH-H) and …

2024年11月28日 · Looking for the definition of FDFHF? Find out what is the full meaning of FDFHF on Abbreviations.com! The Web's largest and most authoritative acronyms and abbreviations resource.

The fdhF gene of Escherichia coli codes for the selenocysteine-including protein subunit of formate dehydrogenase H. The protein subunit consists of 715 amino acid residues containing a single selenocysteine residue at position 140 which is encoded by a UGA codon.

Escherichia coli possesses three distinct formate dehydrogenase enzymes encoded by the fdnGHI, fdhF, and fdoGHI operons.

2014年8月25日 · In this work, genetic tools are reported that allow the facile isolation of FHL in a single chromatographic step. The core complex is shown to comprise HycE (a [NiFe] hydrogenase component termed Hyd-3), FdhF (the molybdenum-dependent formate dehydrogenase-H), and three iron-sulfur proteins: HycB, HycF, and HycG.

2022年9月14日 · We evaluate possible structural determinants of the bias toward hydrogen production over its oxidation and describe an unpredicted metal-binding site near the interface of FdhF and HycF subunits...