Formate dehydrogenases are a set of enzymes that catalyse the oxidation of formate to carbon dioxide, donating the electrons to a second substrate, such as NAD + in formate:NAD+ oxidoreductase (EC 1.17.1.9) or to a cytochrome in formate:ferricytochrome-b1 oxidoreductase (EC …

2020年12月1日 · Formate dehydrogenase (FDH) has been known as an enzyme catalyzing nearly irreversible formate oxidation to CO2 for a long time [4], but recent research has revealed that certain FDHs are capable of reversibly interconverting CO 2 …

2024年7月24日 · Formate dehydrogenases (FDHs) are widely utilized to drive various biocatalysts for the production of valuable compounds. Recent advancements in enzyme engineering have enhanced the functionality of metal-independent FDHs, increasing their applicability and suitability for specific bioprocesses.

2024年2月15日 · Formate dehydrogenase (FDH) is critical for the conversion between formate and carbon dioxide. Despite its importance, the structural complexity of FDH and difficulties in...

2019年11月15日 · We present a new sequence-based classification of FDHs and discuss how the individual FDH enzyme groups are suited for CO 2 reduction. We also examine reaction rates, efficiencies of different common electron donor principles, and …

2023年3月6日 · Based on pioneering work in 1951, the widely accepted Theorell–Chance catalytic mechanism 3, 4, 5, 6 suggests that the catalytic cycle of formate dehydrogenase (FDH) requires the association and...

2023年12月1日 · FDH is an enzyme that catalyses the conversion of formate to carbon dioxide, coupled with the reduction of an electron acceptor such as NAD + or a quinone. Metal ions play a crucial role in the catalytic activity of FDH by assisting in the transfer of electrons and in stabilizing the enzyme structure.

FDH (10-formyltetrahydrofolate dehydrogenase, Aldh1L1, EC 1.5.1.6) converts 10-formyltetrahydrofolate (10-formyl-THF) to tetrahydrofolate and CO 2 in a NADP + -dependent reaction. It is a tetramer of four identical 902 amino acid residue subunits.

2014年12月5日 · To fulfil these varied physiological roles, different types of formate dehydrogenase (FDH) enzymes have evolved to catalyse the reversible 2-electron oxidation of formate to carbon dioxide.

2024年10月9日 · Here we have examined the ability of the FdsDABG formate dehydrogenase from Cupriavidus necator to catalyze the exchange of solvent oxygen into product CO 2 during the course of formate oxidation under single-turnover conditions.