2020年12月1日 · For FDH-driven CO 2 reduction, NADH or other artificial electron mediators have been commonly regenerated in the electroenzymatic system in which NAD + or oxidized artificial electron mediators receive electrons from the cathode and then are

2024年7月24日 · Formate dehydrogenases (FDHs) catalyze the oxidation of formate to CO 2 while reducing NAD (P) + to NAD (P)H and are classified into two main classes: metal-dependent (Mo- or W-containing) and metal-independent FDHs.

2022年2月1日 · Formate dehydrogenases (FDHs) can catalyze reduction of CO2 to HCOO −. Immobilization can improve FDH kinetics for CO 2 reduction. Non-natural electron donors are better than NADH for FDH catalyzed CO2 conversion. Multi-enzyme cascades with FDH have potential for methanol production. Formatotrophic microorganisms can utilize CO 2 via FDH.

2023年10月17日 · Nicotinamide adenine dinucleotide (NADH) and nicotinamide adenine dinucleotide phosphate (NADPH) constitute major hydrogen donors for oxidative/reductive bio-transformations. NAD(P)H regeneration systems coupled with formate dehydrogenases (FDHs) represent a dreamful method.

2023年3月6日 · The combined simulations and experiments demonstrate instead that a fresh NAD + could readily diffuse into the proximity of NADH in FDH, enabling the efficient hydride transfer from the bound...

2014年3月1日 · After previous work on the indirect electro-enzymatic regeneration of NADH, using formate dehydrogenase (FDH) from Candida boidinii free in solution, the present work aimed to drastically reduce the quantity of enzyme required, in order to propose an improved and optimized process.

2024年11月20日 · Formate dehydrogenase (FDH) catalyzes the oxidation of formic acid to CO 2 with the concomitant reduction of nicotinamide adenine dinucleotide (NAD, Fig. 1a) to its...

2010年6月17日 · 摘要：nadh依赖型氧化还原酶广泛应用于精细化学品和手性化合物的生物合成，其中辅酶nadh作为还原当量 起着关键的作用，NADH的再生关系到生物氧化还原过程能否进行.甲酸脱氢酶在辅酶NADH再生中的应用是目前

2024年11月7日 · The bifunctional Rh III-complex concurrently catalyzes gas-phase reduction of CO 2 to formate at 15.8 mM/h and NADH regeneration at 0.24 mM/min. Formate production is 83.2 times faster than using one of the best aerobic FDHs from Clostridium ljungdahlii (ClFDH), resulting in a 3.6-fold increase in methanol production rate (0.43 mM/h) compared ...

Abstract: Formate dehydrogenase (FDH) is a D-2-hydroxy acid dehydrogenase, and catalyzes the oxidation of formate to carbon dioxide, coupled with reduction of NAD + (oxidized nicotinamide adenine dinucleotide (NAD)) to NADH (reduced NAD) that plays a key role in the process of NADH regeneration.