
Structure of the membrane-bound formate hydrogenlyase …
2022年9月14日 · We evaluate possible structural determinants of the bias toward hydrogen production over its oxidation and describe an unpredicted metal-binding site near the interface …
Bacterial formate hydrogenlyase complex - PNAS
2014年8月25日 · In this work, genetic tools are reported that allow the facile isolation of FHL in a single chromatographic step. The core complex is shown to comprise HycE (a [NiFe] …
Coordinate Regulation of the Escherichia coli Formate …
Escherichia coli possesses three distinct formate dehydrogenase enzymes encoded by the fdnGHI, fdhF, and fdoGHI operons. To examine how two of the formate dehyrogenase …
Bioelectrocatalytic carbon dioxide reduction by an engineered …
2024年11月17日 · The electrocatalysis of FdhF_HycB3∆159-184 was characterized by constructing an FdhF_HycB3∆159-184/carbon nanotube/glassy carbon electrode …
Mechanism of Action of Formate Dehydrogenases
2024年10月9日 · It is known that both the molybdenum-containing E. coli FdhF formate dehydrogenase (a component of the organism’s fully reversible formate:hydrogen lyase …
Mutations in trans that affect formate dehydrogenase (fdhF) gene ...
Mutations in fdhS specifically affect fdhF expression without affecting the expression of the other anaerobically induced genes or enzymes that were tested, including hyd, pepT, chlC, nitrite …
Regulation of the fdhF gene encoding the selenopolypeptide for …
The direction of transcription of gene fdhF was found to be counterclockwise on the E. coli chromosome in one Mudl(Aprlac) fusion mutant. Expression of the lac operon in this mutant …
Formate dehydrogenase, ubiquinone, and cytochrome
2020年2月6日 · E. coli also has a third formate dehydrogenase, FDH-H (coded by fdhF), which is a subunit of formate hydrogenlyase, an enzyme complex that disproportionates formate to H 2 …
Anaerobic induction of Escherichia coli formate dehydrogenase ...
In striking contrast to many other anaerobic-specific genes, which require DNA to be negatively supercoiled for expression, we have found that inhibition of DNA gyrase activity enhances …
A Sulfurtransferase Is Essential for Activity of Formate …
2012年2月1日 · FdhF activity is sensitive to cyanide treatment, with the activity being restored with an exogenous sulfur source. Overall, our data support a model in which sulfur transfer …