Sep 14, 2022 · We evaluate possible structural determinants of the bias toward hydrogen production over its oxidation and describe an unpredicted metal-binding site near the interface of FdhF and HycF subunits...

Aug 25, 2014 · In this work, genetic tools are reported that allow the facile isolation of FHL in a single chromatographic step. The core complex is shown to comprise HycE (a [NiFe] hydrogenase component termed Hyd-3), FdhF (the molybdenum-dependent formate dehydrogenase-H), and three iron-sulfur proteins: HycB, HycF, and HycG.

Nov 17, 2024 · The electrocatalysis of FdhF_HycB3∆159-184 was characterized by constructing an FdhF_HycB3∆159-184/carbon nanotube/glassy carbon electrode (FdhF_HycB3∆159-184/CNT/GC).

Escherichia coli possesses three distinct formate dehydrogenase enzymes encoded by the fdnGHI, fdhF, and fdoGHI operons. To examine how two of the formate dehyrogenase operons (fdnGHI and fdhF) are expressed anaerobically in the presence of low, ...

Oct 9, 2024 · It is known that both the molybdenum-containing E. coli FdhF formate dehydrogenase (a component of the organism’s fully reversible formate:hydrogen lyase complex) and the molybdenum-containing Cupriavidus necator FdsDABG formate dehydrogenase catalyze the transfer of the C α hydrogen of substrate to their metal centers in the course of the ...

FdhF interacts with HycB via hydrophobic interactions and hydrogen bonds (H-bonds) (Fig. 4a and Supplementary Fig. 5) and with HycF electrostatically (Fig. 4a, b). Our data suggest that FdhF is more strongly associated with the Hyc subunits under oxidizing than under reducing conditions.

The direction of transcription of gene fdhF was found to be counterclockwise on the E. coli chromosome in one Mudl(Aprlac) fusion mutant. Expression of the lac operon in this mutant was induced by formate and repressed by nitrate, nitrite or trimethylamine N-oxide.

In striking contrast to many other anaerobic-specific genes, which require DNA to be negatively supercoiled for expression, we have found that inhibition of DNA gyrase activity enhances expression from the gene (fdhF) encoding the selenopolypeptide of FDHH.

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The formate dehydrogenase subunit FdhF, which shows partial occu- pancy in the aerobically puri fied complex, was entirely absent in the anaerobically isolated...