2023年9月1日 · FDX1 KO resulted in the suppression of lipid metabolism and cell cycle–related genes and the induction of stress-related gene signatures that include hypoxia-induced genes …

2024年1月22日 · To generate Fdx1-KO murine cells, two single-guide RNA (sgRNA) expression vectors pSpCas9(BB)–2A-Puro-sgFdx1-1 and pSpCas9(BB)–2A-Puro-sgFdx1-2 were used to …

通过免疫荧光也证明了，elesclomol处理显著诱导产生DLAT 小的聚集体（foci），而这种foci在FDX1 KO和脂酰化缺陷细胞中减少(图5F至H)。 Fig. 5. Copper directly binds and promotes the …

2022年12月22日 · We therefore tested if gentle overexpression of FDX2 could functionally complement lipoate deficiency in FDX1 KO cells. We analyzed growth and lipoate production …

研究人员发现铜离子积累主要通过调控 fdx1 来介导铜死亡。 一方面，fdx1 将 cu2+ 还原成更具毒性的 cu+，可促使 tca 循环中硫辛酰化蛋白异常寡聚化，另一方面，fdx1 导致 fe-s 簇蛋白的 …

通过敲除Fdx1基因exon 2，建立Fdx1基因敲除小鼠模型。 *使用本品系发表的文献需注明: Fdx1-KO mice (Cat. NO. NM-KO-232941) were purchased from Shanghai Model Organisms Center, …

2024年12月1日 · Engineered lplA restored lipoylation in all tested lipoylation null cell models, mimicking defects in mitochondrial fatty acid synthesis (MECR KO), Fe–S cluster biosynthesis …

CRISPR/Cas9 to induce FDX1 loss-of-function (we will term FDX1 KO) and controlled for off-target effects by over-expressing FDX1 in the FDX1 loss-of-function cells (KO+OE). These …

FDX1 or lipoyl synthase KO cells, lipoylation in these same cells is not rescued, arguing against an alternative biosynthetic route or salvage pathway for lipoate in hypoxia. Our work re-veals …

We found that the intracellular levels of both cholesterol and TAGs were significantly upregulated in FDX1-KO HCT116 cells compared to isogenic control cells (Figure 4E and F), confirming …