Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications [1][2] including flow cytometry. First described in 1942, [3] FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure.

2024年10月8日 · FAM amine（5-氨基荧光素）和FITC-NH2（异硫氰酸荧光素-氨基）都是绿色荧光染料，具有氨基基团修饰，常用于生物分子标记、检测和成像。 结构特点：FAM amine 是在常见的FAM（5-羧基荧光素）的基础上添加氨基基团修饰。 氨基基团的存在允许它与其他分子（如羧基或其他反应性基团）进行共价结合，便于标记DNA、RNA或蛋白质。 应用：主要用于核酸探针、PCR引物的标记、蛋白质标记等。 由于其稳定的荧光性质，它广泛应用于荧光共振能量转 …

2022年3月17日 · Fluorescein isothiocyanate (FITC) has been extensively used for attaching a fluorescent moiety to proteins, antibodies, and lectins through an amine group. The isothiocyanate functional group reacts with primary amines of proteins at lysine residues and at the protein’s amino terminus.

The isothiocyanate moiety (-N=C=S) on FITC reacts with amino-terminal and primary amine groups on the target biomolecule to form a covalent thiourea bond. The resulting fluorescein conjugates can be used as specific probes in several applications, including enzymatic kinetics, immunocytochemistry, immunohistochemistry, fluorescence microscopy ...

Rapid Reaction with Amines: FITC reacts quickly with amines, allowing for efficient labeling of proteins, antibodies, and other biomolecules. High molecular absorptivity: FITC labels are …

2024年10月16日 · 异硫氰酸荧光素-氨基 (FITC-Amine) 是一种常用的荧光标记试剂，能够通过异硫氰酸基团与氨基的反应，标记蛋白质、抗体、核酸等生物分子。 它广泛用于免疫荧光、流式细胞术、核酸检测和活细胞成像等研究领域，提供了高灵敏度的荧光检测手段。 FITC 的绿色荧光使其成为生物学研究中最常用的标记染料之一。 包装：瓶装. 规格：mg/g. 用途：科研！ 保存时间：一年. 状态：固体/粉末/溶液. 其他： Cy5.5-GGT；荧光染料Cy5.5修饰γ-谷氨酰转肽酶. CY5.5 …

Scheme of FITC labeling peptides on solid support (N-terminal β-alanine). N-terminal amino group can be selectively conjugated with FITC in the presence of the side chain amino groups due to their different pKa values (approx. 8.9 and 10.5, respectively).

2009年1月21日 · Fluorescein isothiocyanate (FITC) is an amine reactive derivative of fluorescein dye that has wide ranging application in biochemistry. It has been extensively used to label peptides and proteins. However, its use in solid phase peptide synthesis is restricted.

Efficiently labels antibodies and other purified proteins at primary amines (lysine side chains). Used in applications including fluorescence microscopy, flow cytometry western blotting and ELISA. 5 (6)-FITC DA (ab145463) precursor. 5-FITC (ab145325) and 6 …

2024年7月28日 · 5-FITC-C2-胺 是一种将异硫氰酸荧光素（FITC）的特性与 C2 位置的氨基相结合的化合物。 生物偶联： C2 位置的氨基提供了一个额外的偶联位点，可与其他生物分子结合，扩大了其在生物偶联化学中的用…