1996年10月1日 · We found that the receptor tyrosine kinase Flk-1, previously shown to be expressed in endothelial cells, is also expressed in neural progenitor cells of the mouse retina. Flk-1 RNA expression in the retinal progenitors commences with the onset of neuronal differentiation and persists throughout retinal neurogenesis.

2000年11月2日 · Here we show that Flk1+ cells derived from embryonic stem cells can differentiate into both endothelial and mural cells and can reproduce the vascular organization process.

2023年8月3日 · Endothelial Flk1 (fetal liver kinase 1) deficiency promotes endothelial dysfunction, vascular remodeling, and pulmonary hypertension in hypoxia (Hx). A, Experimental strategy for Flk1 deficiency–induced pulmonary hypertension (PH) in Hx.

2004年6月15日 · To gain a molecular understanding of the Flk-1 pathway signaling on vascular development, we differentiated in vitro murine embryonic stem (ES) cells into ECs and smooth muscle cells (SMCs) with VEGF-A 165 (further referred to as VEGF-A) in chemically defined serum-free conditions.

Flk1 is the major receptor for VEGF on endothelial cells. During embryogenesis, flk1 is required for both vasculogenesis and angiogenesis and abnormally elevated flk1 expression is often associated with pathological conditions in adults.

In the present study, we combined the KDR/Flk-1-specific neutralizing monoclonal antibody (KDR/Flk-1mAb) and the Retro-Tet system to elucidate the role of KDR/Flk-1 in VEGF-induced tumor development and angiogenesis in a murine HCC experimental model.

During the development of the avascular chick retina, Flk1 mRNA is detected in the proliferative zone of the neural epithelium, whereas the VEGF ligand is expressed by differentiated retinal ganglion cells.

2020年4月22日 · Thus, our findings suggested that Flk1 and Flt1 are predominantly expressed in skin MCs during the maturation process and play a crucial role in the regulation of these MCs. Flk1 is a key regulator of angiogenesis, acting through the promotion of proliferation and survival of the endothelial cells [23, 35, 47, 57].

2016年4月14日 · In this study, we have identified a novel endothelial cell enhancer within the 10th intron of Flk1. Similar to other Flk1 regulatory elements, this enhancer requires both Ets- and Gata-binding motifs for transcriptional activity.

1997年6月13日 · To investigate the role of Flk1 during vasculogenesis and hematopoiesis, we examined the developmental potential of Flk1−/− embryonic stem cells in chimeras. We show that Flk1 is required cell autonomously for endothelial development.