In principle, one mouse must have a tissue-specific driven cre gene and another mouse have loxP flanked (floxed) alleles of interest gene Y. Expression of Cre recombinase excises floxed loci …

Cre小鼠和flox小鼠是两种常用的实验动物模型。 Cre小鼠，就是体内带有 Cre重组酶 基因的小鼠。 Cre是一个细菌的蛋白，可以切割带 LoxP序列 的DNA，LoxP的不同排列也会导致不同的结果 …

Cre-Lox系统的优势在于可以利用组织特异性启动子特异性控制Cre在特定组织的表达，以实现在特定组织细胞中对目的基因的敲除或改造，更好的规避全身敲除小鼠的胚胎致死现象。 1. Cre鼠 …

The Cre/lox system is one of the most powerful and versatile tools developed for mouse genetics. It gives mouse researchers sophisticated control over the location and timing of gene …

2006年5月18日 · Two of the most exciting and versatile genetic tools designed in the last 30 years are the Cre-lox and FLP-FRT technologies. Both allow the location and timing of gene …

Cre-loxP recombination allows scientists to excise, insert, or invert specific DNA segments with unprecedented accuracy. This works through two key components: a Cre recombinase and …

flox/-: 表示该基因座为杂合,一个等位基因带有flox位点,另一个为敲除状态。 flox/flox: 表示该基因座为双重flox,两个等位基因均带有flox位点。 Cre-lox系统 中,flox位点可以使表达Cre酶的组织或 …

lox指的是loxP序列，一段34bp的序列，可以被Cre重组酶（recombinase）识别，然后切上一刀。 最经典最常用的，就是Deletion这个情况：把loxP序列放在想要切掉的基因X两端，当Cre蛋白 …

The Cre-lox system is one of the most revolutionary tools in the geneticist's toolbox. You don't have to be a user or developer of cre or floxed mice to appreciate the impact that cre-lox …

2017年8月11日 · Conditional knockout using Cre/lox is essential for functional analysis of genes. CRISPR/Cas in combination with two sets of guide RNAs and a single-stranded …