In this paper, we propose an efficient self-supervised denoiser Fluorescence Micrograph to Self (FM2S), enabling a high-quality denoised result with a single noisy image.

A single channel image simply means that each pixel has a single intensity value. In fluorescence images, each value represents how much light (or fluorescence) was detected at that point. This website gives a very good simple explanation on how the light detected is transformed into pixels.

Fluorescence images can be provided to the pipeline either as individual grayscale acquisitions or as a single combined color image. There are three possible input modes: One or more acquisitions stored in a single, multi-page TIFF/BigTIFF file, where each page encodes an acquisition in grayscale at 8 or 16-bit depth, up to six total pages.

3 天之前 · To process microscopy images, which mostly have a single channel, we duplicate this channel three times. We found that this approach works well and assume that SAM was also trained on grayscale ...

2022年5月31日 · To circumvent these hardwired biases, single-channel fluorescence images should always be shown in grayscale. This is not to say that coloured images are never useful, however. For example, colocalisation studies are often facilitated by multi-channel overlay images, where analogous colours like red and green are chosen to highlight areas of ...

Single channel grayscale images of different fluorescent probes were combined into RGB images. Image arrangement was as follows: NeuN labeled neurons (red) channel with DAPI stained nuclei (blue) channel and 3-NT (green) channel.

3 天之前 · Most biological processes, from development to pathogenesis, span multiple time and length scales. While light-sheet fluorescence microscopy has become a fast and efficient method for imaging ...

2019年9月2日 · Cytokit is a collection of open source tools for quantifying and analyzing properties of individual cells in large fluorescent microscopy datasets that are often, but not necessarily, generated from multiplexed antibody labeling protocols over many fields of …

Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to distinguish the unique molecular environment of fluorophores. FLIM measures the time a fluorophore remains in an excited state before emitting a photon, and detects molecular variations of fluorophores that are not apparent with spectral techniques alone.

2025年1月29日 · In this letter, we introduce polarized Fourier light field microscopy (pFLFM), a novel fluorescence imaging technique that captures five-dimensional information (3D intensity and 2D polarization) in a single snapshot. This technique combines a polarization camera with an FLFM setup, significantly improving data acquisition efficiency.