Fmoc resin cleavage and deprotection are crucial steps for peptide synthesis, yielding the desired peptide after resin detachment.

通常我们使用的是氨基被Fmoc保护的Sieber-resin，装载第一个氨基酸时应先脱除Fmoc,然后进入正常偶联洗涤脱保护的循环。

2022年3月14日 · A extremely acid-labile resin for preparing peptides and partially protected peptide fragments by the Fmoc strategy. This resin is ideal for use in the preparation of peptides containing C-terminal Cys, His, Met, Tyr and Pro residues. Cleavage for protected peptides from this matrix can be effected by treatment with AcOH/TFE/DCM,0.5% TFA or HFIP.

When making a macrocyclic peptide our first amino acid is almost always Boc-Orn(Fmoc)-OH. Use ca. 100 mg of Boc-Orn(Fmoc)-OH. Using a flow of nitrogen gas, push out all CH2Cl2 from the column that contains the swelled resin and add the amino acid/CH2Cl2/collidine mixture. Rock for at least 8 h (no longer than 24 h).

We present a practical method for the regeneration, loading, and storage of trityl‐chloride resins for the production of hydrazide containing peptides, leveraging 9‐fluorenylmethyl carbazate. We show that these resins are extremely stable under several common resin storage conditions.

Color (visual): white to yellow to beige Appearance of substance (visual): beads Loading (Photometric determination of the Fmoc-chromophore liberated upon treatment with DBU/DMF): 0.40 - 1.00 mmol/g Enantiomeric purity: ≥ 98.0 % (a/a) Swelling Volume (in DMF): lot specific result Identity (of the substitution): passes test Novabiochem Fmoc amino acid: lot number The qualitiy of this resin ...

Pre-loaded resin for synthesis of peptide acids containing a C-terminal phenylalanine amino-acid residue by Fmoc SPPS. This pre-loaded Wang resin is prepared using a synthesis-tested base resin, to ensure reproducibility and efficacy in SPPS. Loading of the Fmoc-amino acid is carried out under conditions to minimize enantiomerization and ...

2025年3月8日 · 2 Results and Discussion 2.1 A Cyclic Acetal-Based Cleavable Linker for Solid Phase Synthesis. As our goal was to develop a recyclable solid support capable of withstanding multiple synthesis and cleavage cycles under standard Fmoc-based SPS conditions, we began by evaluating the suitability of DBA as a cleavable linker for …

2024年7月12日 · Fmoc保护的赖氨酸(Lys)：Fmoc（9-芴甲氧羰基）是一种常用的氨基保护基团，用于保护氨基酸的α-氨基，防止在肽合成过程中发生不必要的反应。 在肽合成的各个步骤中，可以通过酸性或碱性条件去除Fmoc保护基团，从而暴露氨基以继续肽链的延伸。

Fmoc-Ser(Trt)-Wang Resin is a high-quality solid-phase peptide synthesis resin ideal for creating peptides with serine residues. Its unique Fmoc and Trt protection groups ensure efficient synthesis and high purity, making it perfect for pharmaceutical research and drug development.