多肽固相合成法——英文解释： solid phase peptide synthesis 简写为SPPS。 在肽合成的技术方面取得了突破性进展的是R.Bruce Merrifield，他设计了一种肽的合成途径并定名为固相合成途径。

2025年3月6日 · Fmoc Protecting Group. The use of Fmoc chemistry for protection of the alpha amino group has become the preferred method for most contemporary solid and solution phase peptide synthetic processes. Fmoc has also been shown to be more reliable and produce higher quality peptides than Boc chemistry.

1988 Barlos et al. 2-Chlorotritylchloride resin for the Fmoc/tBu SPPS of fully protected peptide fragments, cleavage: AcOH/TFE/ DCM (1:1:3) or HFIP/DCM (1:4) [18]

Today, Fmoc SPPS is the method of choice for peptide synthesis. Very-high-quality Fmoc building blocks are available at low cost because of the economies of scale arising from current multiton production of therapeutic peptides by Fmoc SPPS. Many modified derivatives are commercially available as Fm …

Fmoc protection is especially advantageous in solid-phase peptide synthesis (SPPS), where its compatibility with other reagents and ease of removal streamline synthesis workflows. Upon deprotection, Fmoc yields a byproduct (Dibenzofulvene) that can be monitored by UV spectroscopy, allowing for efficient reaction tracking. [2]

2007年12月13日 · This protocol for solid-phase peptide synthesis (SPPS) is based on the widely used Fmoc/tBu strategy, activation of the carboxyl groups by aminium-derived coupling reagents and...

In this nonspecialist review, we describe the scope and limitations of Fmoc solid-phase peptide synthesis. Furthermore, we provide a detailed protocol for Fmoc peptide synthesis. Keywords: Coupling reagents; Fmoc solid-phase synthesis; Linkers; Resins.

In this nonspecialist review, we describe the scope and limitations of Fmoc solid-phase peptide synthesis. Furthermore, we provide a detailed protocol for Fmoc peptide synthesis.

A wide variety of cysteinyl-protecting groups are available for use in Fmoc SPPS (Solid Phase Peptide Synthesis). The choice depends on the nature of the desired peptide and synthetic strategic. A summary of thiol protecting groups commonly used in Fmoc SPPS (Table 1).

We introduce a protected Fmoc-Dbz (Alloc) base resin that may be directly used for synthesis with most C-terminal amino acids. Following synthesis, quantitative removal of the Alloc group allows conversion to the active N-acyl-benzimidazolinone (Nbz) species, which may be purified and converted in situ to thioester under ligation conditions.