2015年8月4日 · Foci are cells located in a specific organ of the body that are notably different from the surrounding cells. These differences are caused by mutation or other types of cellular damage, and they’re generally the first sign of a developing lesion, tumor or other disease.

Here we aim to review the biological meaning and significance of DNA damage foci, looking specifically at a range of different settings in which such markers of DNA damage and repair are being studied and interpreted.

According to the results, γH2AX and 53BP1 foci were highly consistent after cells exposed to ionizing radiation treatment, they both could reflect accurately the dynamic changes of DSBs damages and repairs; while RAD51 foci only appeared in part of damaged cells, which was consistent with its role mainly involved in homologous recombination ...

One of the experimental techniques for γH2AX detection in cultured cells is immunofluorescent labelling of γH2AX and nuclei followed by microscopy imaging and analysis. In this study, we present the algorithm FoCo for reliable and robust automatic nuclear foci counting in …

Purpose: This protocol describes how to quantify any type of cellular foci, such as phosphorylated Ser139 on histone variant H2A.X (γH2AX) stained images of tissue or cells, using the free NIH Image Processing and Analysis in Java software called Fiji or ImageJ. γH2AX antibody is used to identify DNA damage in the form of double strand breaks by...

In this chapter, we describe a live cell imaging methodology that directly monitors induction and repair of single DSB, recruitment kinetics of DSB repair/sensor factors to DSB sites, and dynamic interaction of DSB repair/sensor proteins with DSBs at single-cell level.

最终实验做出来了，感觉在实验过程中自己总结的经验有以下：荧光易淬灭，暗室操作很重要，在普通荧光显微镜下观察到γH2AX荧光后避光，尽快在共聚焦显微镜下观察拍照；封闭液和二抗的选择很重要，也是早期实验失败的原因；爬片清洗要干净干净，斜面从一侧冲向另一侧，并且及时把积水吸走，保证背景干净。 各位大神，小生也在做这个实验，只有DAPI的荧光，没有γ-H2AX的。 不知道问题出在哪里了。 γ-H2AX是milipore的1：500的稀释。 二抗是中杉金桥的。 还有，我 …

2021年2月5日 · In response to double strand breaks (DSB), repair proteins accumulate at damaged sites, forming membrane-less sub-compartments or foci. Here we explored the physical nature of these foci, using single molecule microscopy in living cells.

2022年9月9日 · The cells stand out from surrounding tissue based on their appearance, special stains, or other testing. Foci is the plural of focus and implies only microscopic visualization of the tumor cells. What is on a chromosome?

DNA damage foci are now widely studied in a range of different research areas, utilizing very diverse organ-isms, such as yeasts, plants, rodents and humans. The fol-lowing sections discuss their meaning and significance in different applications.