2015年8月4日 · Foci are cells located in a specific organ of the body that are notably different from the surrounding cells. These differences are caused by mutation or other types of cellular …

Here we aim to review the biological meaning and significance of DNA damage foci, looking specifically at a range of different settings in which such markers of DNA damage and repair …

According to the results, γH2AX and 53BP1 foci were highly consistent after cells exposed to ionizing radiation treatment, they both could reflect accurately the dynamic changes of DSBs …

One of the experimental techniques for γH2AX detection in cultured cells is immunofluorescent labelling of γH2AX and nuclei followed by microscopy imaging and analysis. In this study, we …

Purpose: This protocol describes how to quantify any type of cellular foci, such as phosphorylated Ser139 on histone variant H2A.X (γH2AX) stained images of tissue or cells, using the free NIH …

In this chapter, we describe a live cell imaging methodology that directly monitors induction and repair of single DSB, recruitment kinetics of DSB repair/sensor factors to DSB sites, and …

最终实验做出来了，感觉在实验过程中自己总结的经验有以下：荧光易淬灭，暗室操作很重要，在普通荧光显微镜下观察到γH2AX荧光后避光，尽快在共聚焦显微镜下观察拍照；封闭液和二抗 …

2021年2月5日 · In response to double strand breaks (DSB), repair proteins accumulate at damaged sites, forming membrane-less sub-compartments or foci. Here we explored the …

2022年9月9日 · The cells stand out from surrounding tissue based on their appearance, special stains, or other testing. Foci is the plural of focus and implies only microscopic visualization of …

DNA damage foci are now widely studied in a range of different research areas, utilizing very diverse organ-isms, such as yeasts, plants, rodents and humans. The fol-lowing sections …