The restriction endonuclease Fok1, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non sequence-specific DNA cleavage domain at the C-terminal. [2]

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Jul 3, 1997 · FokI is a member of an unusual class of bipartite restriction enzymes that recognize a specific DNA sequence and cleave DNA nonspecifically a short distance away from that sequence1,2,3.

FokI can cleave between virtually any two nucleotides by constructing a complementary oligonucleotide to the sequence to be cleaved (Szybalski, W. (1985) Gene 40, 169-173, Podhajska, A. and Szybalski, W. (1985) Gene 40, 175-182).

Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to …

VDR-FokI polymorphism could increase the risk of GC development and predispose to the disease by mechanisms. Key Words: Gastric cancer, PCR-RFLP, Polymorphism, Vitamin D receptor. Introduction. Gastric cancer, as the second most prevalent tumor worldwide, originates from the lining of the stomach and is a significant global threat to public ...

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