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FokI - NEB
An E. coli strain that carries the FokI gene from Flavobacterium okeanokoites (IFO 12536). This product is related to the following categories: Restriction Endonucleases C G
FokI - 百度百科
FokI是一种存在于细菌Flavobacterium okeanokoites的type IIS限制酶，含有位于 N端 的DNA结合区块（N-terminal DNA-binding domain），以及一个位于 C端 的非专一性DNA切割区块。 当 …
FokI - 维基百科，自由的百科全书
FokI 是一種存在於細菌 Flavobacterium okeanokoites 的type IIS限制酶，含有位於 N端 的 DNA結合區塊 （N-terminal DNA-binding domain），以及一個位於 C端 的非專一性DNA切割區塊 [2] …
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基因编辑的原理和载体结构 - 知乎 - 知乎专栏
基因编辑技术是指通过核酸酶对靶基因进行定点改造，实现特定DNA的定点敲除、敲入以及突变等，最终下调或上调基因的表达，以使动植物、细胞等获得新表型的一种新型技术。 目前基因 …
Dimeric CRISPR RNA-guided FokI nucleases for highly specific
2014年4月25日 · Monomeric CRISPR-Cas9 nucleases are widely used for targeted genome editing but can induce unwanted off-target mutations with high frequencies. Here we describe …
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FokI - an overview | ScienceDirect Topics
FokI (Table 1) is an archetype of the Type IIS enzymes. It is a monomeric protein containing two domains connected by a flexible linker: a DNA recognition domain that makes all of the …
Fusion of FokI and catalytically inactive prokaryotic Argonautes ...
2024年9月1日 · In the PNFP system, catalytically inactive pAgo recognizes and binds to a specific target DNA sequence based on a programmable guide DNA sequence; upon binding to the …