FtsI (also called PBP3) of Escherichia coli is a transpeptidase required for synthesis of peptidoglycan in the division septum and is one of several proteins that localize to the septal ring. FtsI comprises a small cytoplasmic domain, a transmembrane helix, a noncatalytic domain of unknown function, and a catalytic (transpeptidase) domain.

2020年5月5日 · This study aimed to characterize the polymorphism of ftsI gene in 19 H. influenzae strains, isolated between 2014 and 2016 (different resistance phenotypes to β-lactams (n = 9) and susceptible strains (n = 10) used for comparative purposes).

2024年12月1日 · The presence of β-lactamase was investigated phenotypically (Cefinase-test, BD, USA) and genotypically (blaTEM, blaROB) by PCR in isolates found positive in benzyl-penicillin-screening test (BPT) in DDm. In BPT-positive isolates, the ftsI gene was amplified by PCR and products were sent to Eurofins-Genomic Lab. (Germany) for sequencing.

2024年9月1日 · One of the mechanisms of resistance to β-lactams is the alteration of the transpeptidase region of penicillin binding protein 3 (PBP3) which is caused by mutations in the ftsI gene. It was shown that exposure to beta-lactams has a stimulating effect on increase of prevalence of H. influenzae strains with the non-enzymatic mechanism of resistance.

FtsI (also called PBP3) of Escherichia coli is a transpeptidase required for synthesis of peptidoglycan in the division septum and is one of several proteins that localize to the septal ring. FtsI comprises a small cytoplasmic domain, a transmembrane helix, a noncatalytic domain of unknown function, …

The most important mechanism for beta-lactam resistance in beta-lactamase-negative ampicillin-resistant (BLNAR) isolates of Haemophilus influenzae is the alteration of penicillin-binding protein 3 (PBP3) as a result of ftsI gene mutations. The present study …

Penicillin-binding protein 3 (PBP 3), which is encoded by the ftsI gene, is a transpeptidase needed for cross-linking of the septal cell wall peptidoglycan during cell division. The essentiality of this enzyme in H. influenzae was demonstrated by targeted gene disruption (18).

1998年8月3日 · In this article, we identify some of the requirements for septal localization of FtsI of E. coli. FtsI (also called penicillin-binding protein 3 [PBP3]) is a bitopic membrane protein with a large periplasmic domain that encodes an enzymatic activity (transpeptidase) involved in peptidoglycan synthesis (1, 10, 42).

2017年9月10日 · The SOS response aids bacterial propagation by inhibiting cell division during repair of DNA damage. We report that inactivation of the ftsI gene product, penicillin binding protein 3, by either β-lactam antibiotics or genetic mutation induces SOS in Escherichia coli through the DpiBA two

The ftsI gene sequences of MDR-AB strain and Acinetobacter baumannii sensitive strain (SDF) were translated into amino acid sequences for homology modeling and visual comparison. Then, the strains were induced with Imipenem,Meropenem and Donipenem, and the expression of ftsI was detected by qRT-PCR.