Design and Use of Fluorescent Fusion Proteins in Cell Biology
The simplest fluorescent fusion proteins (FFPs) are FPs fused to targeting sequences (e.g., a nuclear localization sequence or a signal sequence) for the highlighting of an organelle or a …
Green fluorescent protein: A perspective - PMC - PubMed …
Despite its monolithic appearance, GFP can be expressed as two separate segments that nevertheless combine within cells to yield a functional fluorescent protein. 5,6 This discovery …
Design and applications of a clamp for Green Fluorescent ... - Nature
2017年11月24日 · Green fluorescent protein (GFP) fusions are pervasively used to study structures and processes. Specific GFP-binders are thus of great utility for detection, …
Engineering and characterization of a superfolder green …
2005年12月20日 · Existing variants of green fluorescent protein (GFP) often misfold when expressed as fusions with other proteins. We have generated a robustly folded version of …
Histone–GFP fusion protein enables sensitive analysis of …
1998年3月26日 · We have visualized DMs by developing a highly sensitive method for observing chromosome dynamics in living cells. Results: The human histone H2B gene was fused to the …
Fluorescent Proteins 101: GFP Fusion Proteins - Making the
In this blog I discuss strategies to generate fusion proteins and highlight some aspects of their design. The green fluorescent protein (GFP) from Aequorea victoria and its variants are …
12: GFP fusion proteins - Biology LibreTexts
2024年8月23日 · In order to generate a GFP fusion protein, a cDNA that encodes the protein of interest must be available or obtainable. Then, through molecular cloning, the cDNA for GFP …
超级文件夹绿色荧光蛋白的工程化和表征。,Nature Biotechnology
我们已经生成了一种牢固折叠的gfp版本，称为“超级文件夹” gfp，即使与折叠不良的多肽融合在一起也可以很好地折叠。 与“折叠报告子” GFP（包含“循环3”突变和“增强的GFP”突变F64L …
Green fluorescent protein (GFP) fusion constructs in gene …
GFP can be detected in living cells without selection or staining and it can be fused to other proteins to yield fluorescent chimeras. The potential of GFP has also been recognised by gene …
Purification of GFP fusion proteins with high purity and yield by ...
2008年5月1日 · Here, we demonstrate a novel method, based on high specificity and affinity of monoclonal antibody against GFP, for purification of GFP and GFP fusion proteins. The …