GAL4是酵母（Saccharomyces cerevisiae）转录激活蛋白Gal4的基因。Gal4能识别基因启动子的上游激活序列（UASg）的17bp长的一段序列：5'-CGGRNNRCYNYNYNCNCCG-3'（R表示嘌呤，Y表示嘧啶，N表示任意脱氧核苷酸）。GAL4与GAL80作用，GAL80可结合半乳糖代谢产物。

The Gal4 transcription factor is a positive regulator of gene expression of galactose-induced genes. [1] This protein represents a large fungal family of transcription factors, Gal4 family, which includes over 50 members in the yeast Saccharomyces cerevisiae e.g. Oaf1, Pip2, Pdr1, Pdr3, Leu3. [2]Gal4 recognizes genes with UAS G, an upstream activating sequence, and …

The GAL4-UAS system is a biochemical method used to study gene expression and function in organisms such as the fruit fly. It is based on the finding by Hitoshi Kakidani and Mark Ptashne, [1] and Nicholas Webster and Pierre Chambon [2] in 1988 that Gal4 binding to UAS sequences activates gene expression.

其基本思想是将感兴趣的两个蛋白x和y分别融合到gal4的dbd和ad上，这样x和y之间的相互作用可以重建gal4转录因子的功能，然后驱动报告基因的表达（图1）。在第一个诱饵结构中，蛋白质x（如葡萄糖传感器snf1）被融合到含有dbd的gal4的n端部分（gal4dbd）。

问1. GAL4系统 酵母双杂交的实验原理是什么？. 答1：很多真核生物的位点特异转录激活因子（如GAL4、GCN4）通常具有两个可分割开的结构域，即 DNA特异结合域 （DNA-binding domain, BD）与 转录激活域 （Transcriptional activationdomain, AD）。 将两个待测蛋白分别与这两个结构域建成融合蛋白，并共表达于同一个 ...

2024年6月27日 · 当gal4与uas结合时，uas序列会被激活，从而启动下游基因的转录。 工作原理 1. 构建两个转基因线. gal4驱动线：该线携带gal4基因，其表达受特定启动子控制。例如，假设我们想在果蝇的眼睛细胞中表达特定基因，可以选择与眼睛发育相关的启动子驱动gal4的表达。

2023年10月30日 · 自激活原理：一般情况下GAL4分子的BD可单独与上游激活化序列(upstream activating sequence,UAS)结合，但不能引起转录。 但是某些蛋白本身具有激活或转录功能，使DNA结合结构域杂交蛋白（BD-bait）在无特异激活结构域（AD）的情况下可以激活转录，如果将它构建在BD载体 ...

The GAL4 system is a bipartite system in which one transgenic line, the driver, expresses GAL4 in a known temporal or spatial pattern and a second transgenic line, the responder, contains a UAS-dependent transgene (Fig. 1 A) ().When using the GAL4 system the most important requirement is an appropriate expression pattern of GAL4.

2024年1月1日 · The GAL4/UAS System. There are many tools available when using the fruitfly, Drosophila melanogaster, a model organism highly amenable to genetic analysis.One particular tool is the GAL4/UAS system that allows for the expression of genes from any organism in a tissue and temporal-specific manner.

Abstract. During the past two decades, the yeast Gal4 protein has been used as a model for studying transcriptional activation in eukaryotes. Many of the properties of transcriptional regulation first demonstrated for Gal4 have since been shown to be reiterated in the function of several other eukaryotic transcriptional regulators.