GAPDH with NAD + -binding site mutation competitively inhibits …
Our findings demonstrate that a minimal mutation at the NAD<sup>+</sup>-binding site is sufficient to generate a competitive but dysfunctional GAPDH, and its ectopic expression …
GAPDH with NAD - ScienceDirect
2018年12月1日 · Here, we report that a catalytically-deficient mutant-GAPDH competitively inhibits the wild-type, and disrupts glucose metabolism in cancer cells. Using site-directed …
糖酵解过程及中的重要酶类及其调控（二） - 知乎
催化糖酵解第六步的酶是3-磷酸甘油醛脱氢酶（glyceraldehyde-3-phosphate dehydrogenase，GAPDH）是巯基酶，所以碘乙酸是其不可逆抑制剂。 NAD之间有负协同效 …
（1）GAPDH的功能作用？ - 知乎
突变体GAPDH影响细胞GAPDH的酶促功能，破坏能量代谢。 一般意义：我们的研究结果表明，NAD结合位点的最小突变足以产生竞争性但功能失调的GAPDH，其异位表达抑制野生型破 …
Oxidatively Modified Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH ...
Modification of NAD + is achieved through auto-ADP-ribosylation of cytosolic GAPDH, in which the adenosine 5′-diphosphoribose (ADP-ribose) moiety of NAD + is transferred to another …
GAPDH glyceraldehyde-3-phosphate dehydrogenase [Homo …
2025年3月16日 · The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3 …
Lactate Limits T Cell Proliferation via the NAD(H) Redox State
2020年12月15日 · We now demonstrate that the NAD redox state controls T cell glycolysis via GAPDH, as well as glucose-derived serine production. Our studies open new avenues to …
Engineering of Escherichia coli Glyceraldehyde-3-Phosphate ...
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key enzyme in the central metabolism of microbial cells. GAPDHs differ in cofactor specificity and use NAD +, NADP +, or both …
Disruption of NAD(+) binding site in glyceraldehyde 3-phosphate ...
2015年11月26日 · Site-mutagenesis at positions S98 and T99 in the NAD (+) binding center reduced enzymatic activity of GAPDH due to decreased affinity to NAD (+) (Km = 741 ± 257 …
Rice NAD + -dependent histone deacetylase OsSRT1 represses …
2017年9月13日 · In this work, we show that the rice nuclear sirtuin OsSRT1 not only functions as an epigenetic regulator to repress glycolytic genes expression and glycolysis in seedlings, but …