Glyceraldehyde 3-phosphate dehydrogenase (abbreviated GAPDH) (EC 1.2.1.12) is an enzyme of about 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules.

2023年6月3日 · 与其细胞质形式不同， 细胞核中的GAPDH具有增加的等电点 (pI) pH值为8.3–8.7，并以高特异性结合单链和双链端粒DNA。 GAPDH的过表达已被证明可保护细胞免受诱导鞘脂神经酰胺的化疗药物治疗引发的端粒快速缩短。 随后的研究证实，GAPDH-端粒的直接相互作用是GAPDH对抗神经酰胺诱导的端粒缩短的保护作用所必需的，GAPDH NAD+结合区是端粒DNA结合的位点。 尽管目前仍然存在尚不清楚GAPDH在缺乏神经酰胺的情况下是否在调节端 …

Know comprehensive GAPDH protein information including protein sequence, molecular weight, theoretical pI, structure, function and protein interaction.

GAPDH is most well-known for its role in glycolysis, catalyzing the reversible phosphorylation of G3P to 1,3-bisphosphoglycerate (BPG) using NAD + as a cofactor . The first step of this mechanism involves nucleophilic attack by the sulfhydryl group of Cys-152 on the carbonyl of G3P, resulting in the formation of a hemiacetal .

2013年3月21日 · Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been identified as a key enzyme involved in glycolysis processes for energy production in the Trypanosoma cruzi parasite. This enzyme catalyses the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) in the presence of inorganic phosphat …

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been identified as a key enzyme involved in glycolysis processes for energy production in the Trypanosoma cruzi parasite. This enzyme catalyses the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) in the presence of inorganic phosphate (Pi) an

2018年1月17日 · Analysis of pI of GAPDH might throw light in its relevance and significance in clinical conditions (e.g. Neuro-degenerative diseases, cancer). Hence, immunoblotting under native, non-denaturing condition is critical to determine the …

2014年12月1日 · Structural analysis coordinated with molecular modeling indicates that Akt2-induced phosphorylation of GAPDH thr237 may stearically hinder the formation of the apoptotic GAPDH-Siah1 complex that requires GAPDH lys225.

2006年10月30日 · GAPDH was localized by immunogold labeling and identified by matrix-assisted laser desorption/ionization-time of flight and nano liquid chromatography mass spectroscopy/mass spectroscopy in the...

2016年6月24日 · Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), an important glycolytic enzyme, has a non-catalytic (thus a non-canonical) role in inducing mitochondrial elimination under oxidative stress.