1998年8月7日 · The structural homology between CPC3 and GCN2 called for a closer examination of amino acid regulation incpc-3::hph mutants (abbreviated cpc-3). Starvation for histidine was achieved by supplementing the medium with 3AT, a competitive inhibitor of imidazole glycerophosphate dehydrogenase in histidine biosynthesis ( 68 ).

Half of proteins that are rhythmically expressed under control of the circadian clock in Neurospora crassa are regulated by temporal activity of CPC-3 (the homolog of GCN2) that phosphorylates and inactivates translation initiation factor eIF2α. How …

1998年8月7日 · The 1646 amino acid sequence of this gene, called cpc-3, showed 35% positional identity over almost the entire sequence with GCN2 of yeast, which stimulates translation of the transcriptional activator of amino acid biosynthetic genes encoded by GCN4.

In this study, we discovered that the disruption of the GCN2 (CPC-3) signaling pathway abolished robust circadian rhythms under amino acid starvation, which was important for rhythmic expression of metabolic genes.

sequence of this gene, called cpc-3, showed 35% posi-tional identity over almost the entire sequence with GCN2 of yeast, which stimulates translation of the tran-scriptional activator of amino acid biosynthetic genes encoded by GCN4. Strains disrupted for cpc-3 were un-able to induce increased transcription and derepression

1998年9月1日 · These findings suggest that cpc-3 is the functional homologue of GCN2, being required for increased translation of cpc-1 mRNA in amino acid-starved cells. Molecular organization of the N....

2023年11月11日 · 该研究揭示了粗糙脉孢菌gcn2/cpc-3信号通路感应氨基酸饥饿压力胁迫，并通过重塑染色质调控生物钟基因节律性表达的分子机制，证明了氨基酸代谢与 ...

CPC-3 and CPC-1 are required for robust circadian rhythms under amino acid starvation To investigate whether the nutrient- sensing GCN2 pathway is involved in regulating circadian clock function under amino acid starvation, we created the cpc-3 and cpc-1 knockout mutants (see Mate-rials and methods). In Neurosporacpc-3 and ,

2023年4月21日 · The deletion of GCN2 pathway components disrupts rhythmic transcription of clock gene frq by suppressing WC complex binding at the frq promoter due to its reduced histone H3 acetylation levels. Under amino acid starvation, the activation of GCN2 kinase and its downstream transcription factor CPC-1 establish a proper chromatin state at the frq ...

2023年4月26日 · 近日，中国科学院微生物研究所刘晓研究团队在eLife发表题为“The nutrient-sensing GCN2 signaling pathway is essential for circadian clock function by regulating histone acetylation under amino acid starvation”的研究论文，发现粗糙脉孢菌GCN2信号通路感应氨基酸饥饿压力胁迫，并通过重塑染色质调控生物钟基因节律性表达的分子机制。 正常情况下，生物体的生物钟始终处于一个相对稳定的状态，从而保证生物体生命活动有条不紊的进行。 氨基酸是重 …