GCN4是碱性区-亮氨酸拉链（basic region-leucine zipper）模型中典型的一种。 与其他几种DNA结合蛋白结构域相比，bZIP结构域模型简单清晰，两个结构单元碱性区和拉链区分工明确，既相互依赖又彼此相对独立。 因此，bZIP型结构域是人们开展蛋白质与DNA相互识别、相互作用研究的一个理想的结构域类型。 人们对这一类蛋白的氨基酸对于蛋白与DNA特异性结合的影响进行了广泛且深入的研究。 酵母转录激活因子GCN4（1～281）在酵母细胞中调控氨基酸的生物合成，是 …

2018年4月19日 · We identified 223 genes associated with Gcn4 peaks, in either 5’, ORF, 3’ locations, which, showed significant Gcn4-dependent induction of mRNA as judged by either (i) significantly greater mRNA expression measured in a WT versus gcn4Δ strain both starved for histidine by 3AT treatment (p<0.05) or (ii) significantly greater mRNA expression ...

We report studies of the DNA complex formed by GCN4, a transcriptional activator of eukaryotic amino acid biosynthetic operons. The DNA thermodynamic binding domain, defined by primer extension analysis, spans at least 18 base pairs, a site much larger than the 9-base-pair consensus defined by homology with naturally occurring binding sites.

Analysis of the relative binding constant to the 15 repeated sequences protected by GCN4 shows that the optimal binding site for GCN4 is 5' RRTGACTC 3' followed by a short stretch of thymidines. Another protein, present mostly in yeast nuclear extracts, binds to the HIS4 promoter at a site overlapping one of the GCN4 binding sites.

We conducted a mutational analysis of GCN4 with a single-copy allele expressed under the control of the native promoter and translational control elements. Our results indicate that GCN4 contains two activation domains of similar potency that can function independently to promote high-level transcription of the target genes HIS3 and HIS4.

2017年9月6日 · Our data suggest that Gcn4 is a central regulator of protein synthesis under multiple perturbations, including ribosomal protein gene deletions, calorie restriction, and rapamycin treatment, and...

2011年12月23日 · The structural basis for binding of the acidic transcription activator Gcn4 and one activator-binding domain of the Mediator subunit Gal11/Med15 was examined by NMR. Gal11 activator-binding domain 1 has a four-helix fold with a small shallow hydrophobic cleft at its center.

The unique induction of GCN4 translation in response to eIF2 phosphorylation is mediated by four short open reading frames (uORFs) in the leader of GCN4 mRNA located 150–360 nucleotides upstream of the authentic initiation codon.

We synthesized radioactively pure GCN4 protein by in vitro translation of mRNA produced by in vitro transcription with SP6 RNA polymerase. GCN4 protein binds specifically to the 20 bp region of the HIS3 gene that is critical for transcriptional regulation in vivo and contains the TGACTC sequence common to coregulated genes.

Gcn4 是一种由氨基酸饥饿诱导的酵母转录激活剂。 ChIP-seq 分析显示，在饥饿细胞中，Gcn4 占据了 546 个基因组位点，占 Gcn4 结合基序的 ∼30%。 令人惊讶的是，只有 ∼40% 的结合位点位于启动子中，其中只有 ∼60% 激活转录，表明对 Gcn4 功能进行了广泛的负控制。