Compared with GD2 levels in untreated SH-SY5Y cells, levels of GD2 in SH-SY5Y cells increased 2.5-fold in response to fenretinide under the same conditions used for the GD3 experiments...

2021年1月21日 · Antibodies directed against disialoganglioside (GD2), a TAA found on NBLs, melanomas, and sarcomas, are routinely used in upfront and relapse NBL therapies and have improved...

2021年9月23日 · Here we show that CAR-T cells targeting simultaneously two tumor-associated antigens and providing trans-acting CD28 and 4-1BB co-stimulation, while sharing the same CD3ζ-chain cause rapid...

2015年9月9日 · We found a significant IFNγ release by GD2 CAR T cells over 48-hour cocultures with SH-SY5Y. In addition, we observed that GD2 CAR T cell action was associated with a dramatic release of a potent death ligand, namely TRAIL.

2022年7月11日 · Here, we show that reduced GD2 expression was significantly correlated with the mesenchymal cell state in neuroblastoma and that a forced adrenergic-to-mesenchymal transition (AMT) conferred...

One possible explanation for our findings is that a subset of SY5Y cells may express a level of GD2 just below the threshold antigen density required to trigger killing by the “wild-type” GD2 CAR-engineered T cells. SY5Y exhibits a fairly broad expression pattern spanning greater than 2 logs as measured by flow cytometry (see Fig. 3D ...

In this study, we establish potent synergy for the combination of anti-GD2 and anti-CD47 in syngeneic and xenograft mouse models of neuroblastoma, where the combination eradicates tumors, as well as osteosarcoma and small-cell lung cancer, where the combination significantly reduces tumor burden and extends survival.

2024年4月25日 · The adrenergic cell line SH-SY5Y and its GD2 silenced counterpart SH-SY5Y/GD2 − were employed as controls of expression. Results were expressed as means ± SEM ( n = 3). Subsequently, we investigated the expression of GD3 (GD3S, ST8SIA1) and GD2 (GD2S, B4GALNT1) synthases that are involved in GD2 expression ( 16 ).

2022年7月29日 · 将mes样sh-ep细胞系（gd2低表）与adrn样sh-sy5y细胞系（gd2高表）分别进行抗gd2免疫治疗细胞模型实验，结果显示sh-sy5y细胞被巨噬细胞吞噬及nk介导的肿瘤细胞杀伤作用显著高于sh-ep细胞系，表明在神经母细胞瘤中，细胞状态与gd2的表达和抗gd2治疗的反应有关。

1994年4月14日 · SH-SY5Y cells were found to express at least 12 different gangliosides. RA-induced neuronal differentiation led to a decrease in the content of GM2, GD3, and GD2 and to a 3-7 fold increased concentration of the ganglio-tetraosyl gangliosides GM1, …