2017年6月1日 · Using the spatial intensity distribution analysis (SpIDA), we found that the A206K mutation reduced the dimerization of GFP molecules. Further ectopic expression of Myristoyl-GFP A206K driven by the endogenous AtCLC2 promoter allowed imaging of …

近年来出现的新型wtgfp基因突变体的激发和发射谱发生了改变，热稳定性和荧光强度得到了提高，gfp报告基因在小鼠中的应用就是以这些变体作为基础的。 （4）增强型绿色荧光蛋白（egfp） 现在，应用最为广泛的是红移变体增强型gfp（egfp）。

2015年7月9日 · In GFP-derived FPs, the A206K mutation robustly prevents dimerization at the hydrophobic interface of the β-barrel 21.

2012年8月1日 · The green fluorescent protein (GFP) from the jellyfish Aequoria victoria has been shown to dimerize at high concentrations, which could lead to artefacts in imaging experiments. To ensure a truly monomeric state, an A206K mutation has been introduced into most of its widely used variants, with minim …

Analysis of the fluorescence intensity distribution of GFP ( Figure 1 D) and GFP-A206K ( Figure 1 H) also showed different distributions in stoichiometry, and GFP had two distinct populations...

Plasmid TfRΔEctoR1-eGFP A206K from Dr. Jeanne Stachowiak's lab contains the insert TfRΔEctoR1-eGFP A206K and is published in Biophys J. 2018 Mar 27;114(6):1377-1388. doi: 10.1016/j.bpj.2018.01.036. This plasmid is available through Addgene.

Enhanced GFP (EGFP) rendered monomeric by the A206K mutation.

Using the spatial intensity distribution analysis (SpIDA), we found that the A206K mutation reduced the dimerization of GFP molecules. Further ectopic expression of Myristoyl-GFP A206K driven by the endogenous AtCLC2 promoter allowed imaging of

mEGFP is a basic (constitutively fluorescent) green fluorescent protein published in 2002, derived from Aequorea victoria. It has moderate acid sensitivity. mEGFP was derived from EGFP with the following mutations: A206K amino acid numbers relative to avGFP. show relative to EGFP.

We replaced hydrophobic residues at the crystallographic interface of the dimer with positively charged residues (A206K, L221K, or F223R). These mutants were likewise expressed and purified, and their self-association was measured by analytical ultracentrifugation... We call these novel monomeric GFP variants mCFP, mGFP, and mYFP. These ...