2020年5月7日 · 肌动蛋白是由小球状的G-actin聚合成丝状的F-actin动态交互变化构成的，从而参与了细胞活动中的各种细胞骨架的变化。 通常研究活细胞中actin的活动用的是转染actin-GFP融合蛋白的质粒，可以同时标记F-actin和G-actin，可以有效的反映出细胞骨架的相对活性，但是当值需要观测F-actin的时候，使用actin-GFP会降低信噪比，难以专一捕捉F-actin的活动。 更有研究显示，使用actin-GFP会直接影响细胞迁移和细胞分裂中的肌动蛋白活性。 2015年英国的科研人员 …

To investigate whether the position of the EGFP tag on actin affected the formation of filaments, we constructed an expression vector harboring a β-actin-EGFP gene. In contrast to EGFP-β-actin, cells expressing β-actin-EGFP showed actin filaments in a …

Image actin dynamics in live cells with CellLight Actin-GFP. This ready-to-use fluorescent protein-based reagent contains an actin targeting sequence fused to emerald GFP (emGFP) and utilizes the powerful BacMam technology for delivery.

2016年1月25日 · Recently LifeAct-GFP (green fluorescent protein) has been proposed for visualising actin structure and dynamics in live cells as an alternative to actin-GFP which has been shown to affect cell mechanics. Here we compare the two approaches in terms of their effect on cellular mechanical behaviour.

2004年12月10日 · To investigate the dynamic nature of the actin cytoskeleton, we used a fusion protein between green fluorescent protein (GFP) and the second actin-binding domain (fABD2) of Arabidopsis (Arabidopsis thaliana) fimbrin, AtFIM1. The GFP-fABD2 fusion protein labeled highly dynamic and dense actin networks in diverse species and cell types, revealing ...

2017年2月1日 · Referring to the gold standard F-actin marker phalloidin to stain actin in fixed samples and tissues, we highlight methods for visualization of actin in living cells, which mostly apply the principle of genetically fusing fluorescent proteins to different actin-binding domains, such as LifeAct, utrophin and F-tractin, as well as anti-actin ...

The product of a GFP-actin gene fusion, permanently or transiently transfected in diverse mammalian cell lines, was shown to be a suitable, intrinsic probe of both the organization and dynamics of the actin cytoskeleton. In live Swiss 3T3 and NIH 3T3 cells, the fusion protein was found to accumulate …

T cell signaling is inextricably linked to actin cytoskeletal dynamics at the immunological synapse (IS). This process can be imaged in living T cells expressing GFP actin or fluorescent F-actin binding proteins.

To investigate whether the position of the EGFP tag on actin affected the formation of filaments, we constructed an expression vector harboring a β-actin-EGFP gene. In contrast to EGFP-β-actin, cells expressing β-actin-EGFP showed actin filaments in a …

Here we demonstrate that expression of GFP-mTalin causes severe defects in actin organization and cell expansion in Arabidopsis (Arabidopsis thaliana) root hairs and that GFP-mTalin inhibits the actin depolymerizing activity of the endogenous actin binding protein ADF in vitro.