To better understand the molecular and phenotypic effects of dsRNA-GFP in honey bees and to evaluate its use as a control for RNAi studies, we examined the impact of dsRNA-GFP on global gene expression patterns in developing workers. The dsRNA-GFP was introduced using a non-invasive feeding protocol [23].

2023年5月20日 · Nosema ceranae is an intracellular parasite invading the midgut of honeybees, which causes serious nosemosis implicated in honeybee colony losses worldwide. The core gut microbiota is...

2020年1月31日 · To test whether engineered S. alvi robustly colonizes bees, we inoculated newly emerged, antibiotic-treated bees en masse with S. alvi transformed with a plasmid expressing …

2019年5月14日 · Here, we report on horizontal RNA flow among honey bees mediated by secretion and ingestion of worker and royal jelly diets. We demonstrate that transmission of jelly-secreted dsRNA to larvae is biologically active and triggers gene …

We engineered a symbiotic bee gut bacterium, Snodgrassella alvi, to induce eukaryotic RNA interference (RNAi) immune responses. We show that engineered S. alvi can stably re-colonize bees and produce double-stranded RNA to activate RNAi and repress host gene expression, thereby altering bee physiology, behavior, and growth.

2020年2月17日 · After establishing that engineered S. alvi could colonize bee guts, the authors inoculated bees with S. alvi expressing plasmid-encoded dsRNA against green fluorescent protein (GFP;...

2021年4月26日 · The removal of mite-infested pupae by worker bees, Varroa Sensitive Hygiene (VSH), leads to cannibalization of pupae with high DWV loads, thereby offering an alternative route for virus...

2013年1月4日 · Here, we performed three independent experiments using microarrays to examine the effect of dsRNA-GFP treatment (introduced by feeding) on global gene expression patterns in developing worker bees. Our data revealed that the expression of nearly 1,400 genes was altered in response to dsRNA-GFP, representing around 10% of known honey bee genes.

2023年6月12日 · A strain of a specialized honey bee gut symbiont was genetically engineered to activate the RNA interference system of Nosema to speciically suppress parasite genes essential to the infection. The engineered symbionts colonized bee guts, inhibited Nosema proliferation, and increased the survivorship of bees challenged with parasite infection.

2017年4月26日 · In our experiments Lactobacillus plantarum strain B35, isolated from honey bee digestive tract, was modified using pAD43-25 plasmid carrying a functional GFP gene sequence (gfpmut3a) and used as a model for monitoring and optimisation of the mode of application.