2020年9月4日 · Here we show that, in cryocut sections of snap-frozen brain tissue from two GFP reporter mouse lines, leaking of the GFP signal is prevented by omitting the commonly performed drying of the...

2016年6月17日 · In this work we present whole brain imaging of adult rat brain that has been cleared using the FluoClearBABB method, based on dehydration in increasing concentrations of tert-butanol 10. For...

1998年12月15日 · We demonstrate here that GFP is expressed selectively by subsets of neurons in the brain and spinal cord in adult and developing brain. In cells that strongly express GFP, their entire dendritic and axonal arbor is labeled.

2014年1月7日 · Therefore, we investigated oral delivery of green fluorescence protein (GFP) or myelin basic protein (MBP) fused with the transmucosal carrier cholera toxin B subunit (CTB), expressed in chloroplasts (bioencapsulated within plant cells) to the brain and retinae of triple transgenic Alzheimer's disease (3×TgAD) mice, across the blood–brain ...

2021年2月15日 · In this study, we report a novel inhibitory neuron-specific promoter. Intravenous infusion of the blood–brain barrier (BBB)-penetrating AAV-PHP.B-expressing enhanced green fluorescent protein (GFP) under the control of the new promoter achieved highly efficient and widespread labeling of inhibitory neurons throughout the brain.

2012年8月24日 · New tools have emerged to help identify neurons in the myriad of brain neurons by expressing green fluorescent protein (GFP) under the control of a particular promoter. To monitor both spatially and temporally stimulus-induced Ca 2+ responses in GFP-tagged neurons, a non-green fluorescent Ca 2+ indicator dye needs to be used.

3 天之前 · We noted that the expression level varies across different organs, with the highest GFP signal detected in the brain for both enAsCas12a and LbCas12a mice.

2020年1月9日 · This report describes the generation of a mouse line allowing the detection of single brain cells synthesizing BDNF. A bicistronic construct encoding BDNF tagged with a P2A sequence preceding GFP allows the translation of BDNF and GFP as separate proteins.

This study highlights the value of ubiquitin C/green fluorescent protein (UBC-GFP) transgenic zebra finches in studying adult neurogenesis and advances our understanding of dispersed long-distance neuronal migration in the adult brain, shedding light on this understudied phenomenon.

2023年1月17日 · To confirm the expression of ChAT-eGFP in the entire mouse brain, here we report the precise qualitative distribution of neurons that express the inserted eGFP and compare that distribution to the traditional immunohistochemical method of labeling ChAT, as well as with the in situ hybridization (ISH) of the ChAT mRNA expression published by the ...