SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Vector for fusing EGFP to the N-terminus of a partner protein. For other reading frames, use pEGFP-C2 or pEGFP-C3.

pEGFP-C1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;emission maximum = 507 nm.) pEGFP-C1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

EGFP C1, pEGFPC1, EGFPC1 Plasmid Type Mammalian Expression Promoter CMV Expression Level High Cloning Method Restriction Enzyme Size 4731 5' Sequencing 1 Primer EGFP-C 5' Sequencing 1 Primer Sequence CATGGTCCTGCTGGAGTTCGTG 3' Sequencing 1 Primer SV40pA-R 3' Sequencing 1 Primer Sequence GAAATTTGTGATGCTATTGC Tag 1

2015年3月12日 · Plasmid GFP-C1-PLCdelta-PH from Dr. Tobias Meyer's lab contains the insert PLCdelta and is published in Curr Biol. 1998 Mar 12. 8(6):343-6. This plasmid is available through Addgene.

Plasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418.

Vector for fusing AcGFP1 to the N-terminus of a partner protein. For other reading frames, use pAcGFP1-C2 or pAcGFP1-C3. Sequence Author: Clontech (TaKaRa) Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. PciI is inhibited by nonionic detergents. Sticky ends from different BsaI sites may not be compatible.

The pEGFP-C1 vector features an enhanced green fluorescent protein (EGFP) gene. Its multiple cloning site is located after the GFP gene, enabling the N-terminus of the target protein to be fused with GFP in the constructed fusion protein. Commonly used in molecular biology research, it offers a useful tool for observing protein localization and ...

pEGFP-C1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-C1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

The pEGFP-C1 vector features an enhanced green fluorescent protein (EGFP) gene. Its multiple cloning site is located after the GFP gene, enabling the N-terminus of the target protein to be fused with GFP in the constructed fusion protein. Commonly used in molecular biology research, it offers a useful tool for observing protein localization and ...

pEGFP-C1编码野生型GFP（1-3）的红移变体，该变体已经过优化，可以在哺乳动物细胞中获得更亮的荧光和更高的表达。 （最大激发波长= 488 nm；最大发射波长= 507 nm。 ）pEGFP-C1编码GFPmut1变体（4），其中含有Phe-64取代为Leu和Ser-65取代为Thr的双氨基酸。 EGFP基因的编码序列包含超过190个沉默碱基的变化，这些变化对应于人类密码子使用偏好（5）。 EGFP侧翼的序列已转化为Kozak共有翻译起始位点（6），以进一步提高真核细胞的翻译效率。 pEGFPC1 …