The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. [2] [3] The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP.

1996年2月1日 · The simplest way to shift the emission color of GFP is to substitute histidine or tryptophan for the tyrosine in the chromophore, but such blue-shifted point mutants are only dimly fluorescent. The longest wavelengths previously reported for the excitation and emission peaks of GFP mutants are 488 and 511 nm, respectively.

Fluorescent proteins emitting in the blue region of the visible light spectrum (approximately 440 to 470 nanometers) were first obtained from site-directed mutagenesis efforts targeted at the tyrosine amino acid residue at position 66 in the GFP chromophore (see Figure 2).

2014年8月13日 · Therefore, we report the development and characterization of fast folder and thermo-tolerant green variant (FF-GFP), and a fast folder thermostable yellow fluorescent protein (FFTS-YFP) endowed with remarkably improved thermostability and folding kinetics.

GFP’s versatility and diverse color range enable its application in new research contexts, taking biotechnological studies to new heights. Since GFP responds to energetic excitation by producing a bright light, GFP is ideal as a fluorescent tag .

2008年10月8日 · Today all the colours of the rainbow can be found in a range of GFP and GFP-like proteins. Apart from the obvious biomedical implications, GFP sensors have also been developed that can detect...

This review is an attempt to characterize the main color groups of GFP-like proteins, describe their structure and mechanisms of chromophore formation, systemize data on their conformational stability and summarize the main trends of their utilization as markers and biosensors in cell and molecular biology.

Invitrogen Green Fluorescent Protein (GFP) is a useful expression label in the blue channel for flow cytometry and imaging applications. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm.

The availability of genetically encoded labels such as green fluorescent protein (GFP) offers unique opportunities by which to trace cell movements, cell signaling or gene expression dynamically in developing embryos.

These include five color classes (cyan, green, yellow, and two red classes), a class of colorless proteins found in the extracellular protein matrix of animals, including humans, that lack a chromophore entirely 51 and a class of nonfluorescent chromoproteins 52 that provide deep purple or blue coloration. More classes probably remain to be ...