Escherichia coli GFP - 25922GFP - ATCC
This clone was derived from ATCC 25922 and contains a multicopy vector encoding the green fluorescent protein (Aequorea victoria) GFPmut3. This gene is expressed under the control of …
Green Fluorescent Protein Functions as a Reporter for Protein ...
The use of green fluorescent protein (GFP) as a reporter for protein localization in Escherichia coli was explored by creating gene fusions between malE (encoding maltose-binding protein …
GFP Gene Cloning and E. coli: Techniques and Applications
2025年1月10日 · Explore the methods and applications of GFP gene cloning in E. coli, focusing on expression vectors, protein stability, and fluorescence detection. Green Fluorescent Protein …
Molecular Cloning of Green Fluorescent Protein in Escherichia coli
2013年4月1日 · We ran PCR on protein coding DNA (GFP), cloned GFP into plasmids and transformed the plasmids into E. coli cell by varying the number of templates, and increased …
Green fluorescent protein-expressing Escherichia coli as a
2006年10月24日 · Recombinant Escherichia coli (E. coli) bacteria expressing green fluorescent protein (GFP) was used as a model system to investigate the antimicrobial activities of Ag …
Display of green fluorescent protein on Escherichia coli cell …
2001年1月2日 · In this study, expression of green fluorescence protein (GFP) on the external surface of Escherichia coli was achieved by construction of a fusion protein between Lpp …
Green Fluorescent Protein-based Expression Screening of …
In the protocol described in this article, ligation independent cloning into a GFP reporter vector is combined with fluorescence detection to rapidly screen the relative expression of multiple …
Engineering and characterization of a superfolder green fluorescent protein
2005年12月20日 · Wild-type green fluorescent protein (GFP) misfolds when expressed in E. coli 1. Better-folded variants 1, 2, 3 of GFP are widely employed as protein fusion tags...
Display of green fluorescent protein on Escherichia coli cell …
2001年1月2日 · In this study, expression of green fluorescence protein (GFP) on the external surface of Escherichia coli was achieved by construction of a fusion protein between Lpp …
Green fluorescent protein is a quantitative reporter of gene …
Numerous studies suggest that GFP may be useful as a quantitative reporter of gene expression. Lissemore et. al. describe methods for measuring GFP expression in E. coli cells grown on …