
Expanding the application of a UV-visible reporter for ... - Nature
2021年11月1日 · Here, we report the application of a GFP-like protein, called eYGFPuv, in both transient expression and stable transformation, in two herbaceous plant species (Arabidopsis and tobacco) and two...
pAXY0001 - Addgene
Plasmid pAXY0001 from Dr. Xiaohan Yang's lab contains the insert eYGFPuv and is published in Hortic Res. 2021 Nov 1;8(1):234. doi: 10.1038/s41438-021-00663-3. This plasmid is available through Addgene.
妙啊!没有"火眼金睛"也可以看见的遗传转化! - 知乎
eYGFPuv是一种新型绿色荧光蛋白基因,它在紫外光的激发下可表现出明亮的荧光,在转基因植物中肉眼就可以观察到其稳定表达。将eYGFPuv转到矮牵牛中,结果发现,该蛋白不仅具有强烈、稳定的荧光,而且不需要滤光片,裸眼即可观察(图8-10)。
Generation of brilliant green fluorescent petunia plants by …
2018年11月8日 · Here, to overcome this limitation, we generated transgenic petunia plants expressing eYGFPuv, a CpYGFP derivative exhibiting bright fluorescence under invisible ultraviolet (UV) light excitation,...
高效GFPuv荧光筛选基因编辑载体的改造及其在马铃薯遗传转化中 …
Recent studies revealed that an enhanced Yellow Green Fluorescent like Protein (eYGFPuv (GFPuv)) obtained by mutation can emit strong and stable green fluorescence under 365 nm UV light irradiation and be easily observed.
GFP及其衍生型荧光蛋白、突变体的详细介绍 - 知乎
近年来出现的新型wtgfp基因突变体的激发和发射谱发生了改变,热稳定性和荧光强度得到了提高,gfp报告基因在小鼠中的应用就是以这些变体作为基础的。 (4)增强型绿色荧光蛋白(egfp) 现在,应用最为广泛的是红移变体增强型gfp(egfp)。
Expanding the application of a UV-visible reporter for ... - PubMed
2021年11月1日 · Here, we report the application of a GFP-like protein, called eYGFPuv, in both transient expression and stable transformation, in two herbaceous plant species (Arabidopsis and tobacco) and two woody plant species (poplar and citrus).
植物表达这个GFP,荧光肉眼可见_研究 - 搜狐
2021年1月3日 · 绿色荧光蛋白基因(Green fluorescent protein,简称GFP)是分子生物学研究中常用的报告基因,是一个由约238个氨基酸组成的蛋白质,从蓝光到紫外线都能使其激发,发出绿色荧光,在…
eYGFPuv :: Fluorescent Protein Database
eYGFPuv is a basic (constitutively fluorescent) long stokes shift fluorescent protein published in 2017, derived from Chiridius poppei. It has very low acid sensitivity.
有用!新型报告系统RUBY:可见光下裸眼无损观察植物遗传转化和 …
目前,已有多种报告基因系统,如 荧光蛋白 (gfp、rfp和cfp等)、 β-葡糖醛酸糖苷酶 (gus)以及 荧光素酶 (luc)被开发出来。需要指出的是,这些系统一方面必须借助特殊仪器设备(荧光激发器)或昂贵的底物(x-gluc和萤光素等)才得以观察,另一方面难以 ...