We demonstrate the utility of five fluorescent proteins with optimal characteristics for multicolor experiments, three of which can be easily used simultaneously (GFP or Venus, mTagBFP2 …

2014年1月1日 · Green fluorescent protein (GFP) is an ideal reporter in in vivo studies. Flow cytometry and fluorescent microscopy are two conventional tools to detect the GFP signal; …

Invitrogen Green Fluorescent Protein (GFP) is a useful expression label in the blue channel for flow cytometry and imaging applications. GFP can be excited by the 488 nm laser line and is …

Early studies using FPs in flow cytometry include the quantitative detection and monitoring of gene expression in yeast [2], bacteria [3], and eukaryotic cells [4]. Today there are over 70 …

2024年11月1日 · In this report we demonstrated that the 405-nm violet laser excitation could be used to discriminate GFP and YFP signals. Using the violet laser to distinguish GFP and YFP …

1999年8月1日 · Simultaneous detection of GFP fluorescence and DNA content by PI staining is possible following transient transfection of cells with a single expression vector encoding GFP. …

Fluorescent proteins are now a critical tool in all areas of biomedical research. In this article, we review the techniques required to use fluorescent proteins for flow cytometry, concentrating …

Flow cytometry and fluorescent microscopy are two conventional tools to detect the GFP signal; flow cytometry is an effective and sensitive technique to quantitatively analyze fluorescent …

2017年9月1日 · Here, we present a simple, rapid and robust flow cytometric method that can be used as a tool to optimize transfection efficiency at the single cell level while overcoming …

2004年6月29日 · In this report, we describe a novel flow cytometric method for evaluating the viability of bacterial cells using a combination of GFP and PI. Our results are similar to those of …