2020年9月4日 · We show that fixation methods crucially affect the quality and the localization of the GFP signal in unfixed, snap-frozen tissue. By using the Cx3cr1-GFP reporter mouse 12, we also show that...

There are many techniques to utilize GFP in a live cell imaging experiment. The most direct way of utilizing GFP is to directly attach it to a protein of interest. For example, GFP can be included in a plasmid expressing other genes to indicate a successful transfection of a gene of interest.

2019年9月22日 · GFP是什么？GFP 绿荧光蛋白(Green Fluorescent Portein，GFP)绿色荧光蛋白是一类存在于包括水母、水螅和珊瑚等腔肠动物体内的生物发光蛋白，当受到紫外或蓝光激发时，发射绿色荧光。

1 天前 · Visualizing GFP expression in live primates requires imaging techniques that capture fluorescence without disrupting physiological processes. Confocal and two-photon microscopy provide high-resolution imaging of GFP-expressing cells in excised tissues or superficial layers. Two-photon microscopy allows deeper tissue penetration due to longer ...

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria can be used as a genetically encoded fluorescence marker due to its autocatalytic formation of the chromophore. In recent years, numerous GFP-like proteins with emission colors ranging from cyan to red were discovered in marine …

2024年12月18日 · Here, we present single-molecule tracking datasets for chromatin-bound histone H3 (Hht1) in live and fixed cells and freely diffusing GFP in S. pombe nuclei to benchmark the diffusion...

绿色荧光蛋白 （GFP）等蛋白质的生物发光和荧光可能在水母等生物中存在了数百万年；然而，直到20世纪60年代，科学家们才开始研究GFP并推断其生化特性。 现在，GFP及其荧光衍生物已成为实验室的主要研究对象。 GFP被广泛应用于生物学领域的研究，科学家们利用GFP实现多种功能，包括：标记基因以阐明其表达或定位特征，充当生物传感器或细胞标记，研究蛋白质之间的相互作用，可视化启动子的活动，等等。 为什么是绿色荧光蛋白？ GFP是一种约27kda的蛋白 …

20 小时之前 · Fig. 1: Fast imaging of live cell anatomy of 15 intracellular structures by the universal lipid staining and deep learning segmentation using a spinning disk confocal microscope with an extended ...

中克隆的一种与绿色荧光蛋白(gfp)同源的荧光蛋白，在紫外光的照射下可发射红色荧光，有着广泛的应用前景;但它自身的缺点如寡聚化、成熟缓慢等限制了它的进一步应用。

2025年1月18日 · GFP integration into molecular biology has opened a window into dynamic cellular processes. Researchers use its fluorescent properties to study gene expression in real-time, providing an unprecedented view of cellular activities. GFP can be used as a reporter gene, offering insights into promoter activity and gene regulation.