为寻找激活褐色脂肪细胞的小分子物质，研究人员在褐色脂肪成熟标志物解偶联蛋白 (UCP1)终止密码子前定点敲入绿色荧光蛋白 (GFP)表达框，建立了成熟褐色脂肪组织细胞荧光示踪的小鼠模型（如图4所示）。 随后作者喂食荧光示踪小鼠1000余种临床用药通过观察荧光强度来检测体内Ucp1基因的表达水平，结果发现sutent能显著促进褐色脂肪细胞中UCP1的表达。 本研究结合荧光标记的基因示踪小鼠模型和大规模化合物筛选，建立了一个筛选调控褐色脂肪组织细胞激活剂 …

Certain hematopoietic cell types display distinct expression levels of GFP, allowing identification of different cells types by FACS analysis. This strain is a useful tool for studying hematopoietic cell differentiation and in vivo leukocyte tracking.

These mice may be useful in studying thymocyte positive- and negative-selection as well as lymphocyte activation. 016617 - Nur77[GFP] Strain Details If you need help with your order, contact Customer Support

We have generated transgenic mice that express green fluorescent protein (GFP) under the control of the mouse insulin I gene promoter (MIP). The MIP-GFP mice develop normally and are indistinguishable from control animals with respect to glucose …

1998年8月1日 · EGFP (enhanced GFP) is one such wtGFP variant. As a first step in assessing the use of EGFP in ES cell-mediated strategies, we have established a mouse embryonic stem (ES) cell lines expressing EGFP, which can be propagated in culture, reintroduced into mice, or induced to differentiate in vitro, while still maintaining ubiquitous EGFP expression.

Photoactivation enables rapid, stable fluorescent labeling of living cells with great microanatomical precision.

2015年11月2日 · To facilitate their study, we generated a mouse bearing enhanced green fluorescent protein (EGFP)-specific CD8 + T cells. Transfer of the T cells into EGFP reporter animals can be used to kill...

该小鼠带有CAG启动子驱动的eGFP荧光基因,可在全身各个脏器稳定表达荧光基因。 *使用本品系发表的文献需注明: CAG-GFP-Tg mice (Cat. NO. NM-TG-00005) were purchased from Shanghai Model Organisms Center, Inc.. Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。 常见的基因工程小鼠可以分为两种命名方式，包括基因定点 …

1 天前 · The GFP signal level of splenocytes corresponded well with the copy number of enAsCas12a transgene (Supplementary Fig. 1i,j), while LSL-enAsCas12a, enAsCas12a-heterozygote and homozygote mice did ...

标记基因绿色荧光蛋白(gfp)功能： 当绿色荧光蛋白的基因和目的基因相融合时，蛋白质既能保持其原有的活性，绿色荧光蛋白的发光能力也不受影响。 通过显微镜观察这种发光的“标签”，科学家就能做到对蛋白质的位置、运动、活性以及相互作用等一目了然。